Next Article in Journal
Effects of Microcystin-LR on the Microstructure and Inflammation-Related Factors of Jejunum in Mice
Next Article in Special Issue
Influence of Two Garlic-Derived Compounds, Propyl Propane Thiosulfonate (PTS) and Propyl Propane Thiosulfinate (PTSO), on Growth and Mycotoxin Production by Fusarium Species In Vitro and in Stored Cereals
Previous Article in Journal
Immunohistochemical Review of Leydig Cell Lesions in Ochratoxin A-Treated Fischer Rats and Controls
Previous Article in Special Issue
Biological Control of Citrus Postharvest Phytopathogens
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Toxins
Volume 11
Issue 8
10.3390/toxins11080481
Review Report
toxins-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

Printed Edition

A printed edition of this Special Issue is available at MDPI Books....
share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Biotransformation of the Mycotoxin Zearalenone to its Metabolites Hydrolyzed Zearalenone (HZEN) and Decarboxylated Hydrolyzed Zearalenone (DHZEN) Diminishes its Estrogenicity In Vitro and In Vivo

Toxins 2019, 11(8), 481; https://doi.org/10.3390/toxins11080481
by Sebastian Fruhauf 1, Barbara Novak 1, Veronika Nagl 1,*, Matthias Hackl 2, Doris Hartinger 1, Valentina Rainer 1, Silvia Labudová 1, Gerhard Adam 3, Markus Aleschko 1, Wulf-Dieter Moll 1, Michaela Thamhesl 1 and Bertrand Grenier 1
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Toxins 2019, 11(8), 481; https://doi.org/10.3390/toxins11080481
Submission received: 26 July 2019 / Revised: 9 August 2019 / Accepted: 16 August 2019 / Published: 20 August 2019
(This article belongs to the Special Issue Novel Approaches to Minimising Mycotoxin Contamination)

Round 1

Reviewer 1 Report

Use cell proliferation or survival instead WST-1 as keyword.

The figure 2 doesn’t show the effect of estradiol on cell proliferation. 

Authors assessed the effects of ZEN and etc. on MCF-7 cell lines but evaluated their in vivo effects  on uterus. Could you explain the discrepancy?

It is currently accepted that estrogens play a pivotal role also in males. Please read the following manuscript. doi: 10.3389/fonc.2018.00002.

 

What kind of enzyme the authors suppose to use to modify ZEN?

The possible use of enzymes to inactivate ZEN seems not available.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

Authors studied estrogenic activity of zearalenone and its metabolites using two in vitro assays and in vivo assay and demonstrated that the two metabolites of zearalenone have no estrogenic potency. Expression of microRNAs is hard to use for the detection of estrogenic activity at present.
For all the study is reasonably simple and easy to understand, therefore, I have no critical comments. Only one important future direction, based on these studies, need to be discussed in the last part of this manuscript. How to reduce estrogenicity (or how to metabolize zearalenone) in foods, feeds using degradation enzymes authors proposed. This is the objective of this study, therefore, authors may need to discuss this point. 

Minor editorial sug50, 56, 57, 60, 61, 63, gestions are as follows:

line 13: in vitro should be italic References should be checked: journal name should be appropriately abbreviated, ref no. 2, 5, 7, 8, 9, 11, 12, 17, 19, 21, 23, 24, 26, 30, 38, 41, 44, 45 Ref. 67, 68: Are these references correct? Ref. 32: Some authors name (Onose, Yamaguchi, Osada) should be revised.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

The current form of the manuscript is suitable for publication in Toxins.

Back to TopTop