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Toxins 2019, 11(4), 214; https://doi.org/10.3390/toxins11040214

Fluorescence Spectroscopic Investigation of Competitive Interactions between Quercetin and Aflatoxin B1 for Binding to Human Serum Albumin

1
College of Food Science, Southwest University, Chongqing 400715, China
2
Biological Science Research Center, Southwest University, Chongqing 400715, China
*
Author to whom correspondence should be addressed.
Received: 28 February 2019 / Revised: 2 April 2019 / Accepted: 3 April 2019 / Published: 9 April 2019
PDF [1138 KB, uploaded 9 April 2019]
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Abstract

Aflatoxin B1 (AFB1) is a highly toxic mycotoxin found worldwide in cereals, food, and animal feeds. AFB1 binds to human serum albumin (HSA) with high affinity. In previous experiments, it has been revealed that reducing the binding rate of AFB1 with HSA could speed up the elimination rate of AFB1. Therefore, we examined the ability of quercetin to compete with AFB1 for binding HSA by fluorescence spectroscopy, synchronous spectroscopy, ultrafiltration studies, etc. It was shown that AFB1 and quercetin bind to HSA in the same Sudlow site Ӏ (subdomain IIA), and the binding constant (Ka) of the quercetin-HSA complex is significantly stronger than the complex of AFB1-HSA. Our data in this experiment showed that quercetin is able to remove the AFB1 from HSA and reduce its bound fraction. This exploratory work may be of significance for studies in the future regarding decreasing its bound fraction and then increasing its elimination rate for detoxification. This exploratory study may initiate future epidemiological research designs to obtain further in vivo evidence of the long-term (potential protective) effects of competing substances on human patients.
Keywords: aflatoxin B1; quercetin; human serum albumin; competitive interaction; fluorescence spectroscopy aflatoxin B1; quercetin; human serum albumin; competitive interaction; fluorescence spectroscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Tan, H.; Chen, L.; Ma, L.; Liu, S.; Zhou, H.; Zhang, Y.; Guo, T.; Liu, W.; Dai, H.; Yu, Y. Fluorescence Spectroscopic Investigation of Competitive Interactions between Quercetin and Aflatoxin B1 for Binding to Human Serum Albumin. Toxins 2019, 11, 214.

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