15N Stable Isotope Labeling PSTs in Alexandrium minutum for Application of PSTs as Biomarker
AbstractThe dinoflagellate Alexandrium minutum (A. minutum) which can produce paralytic
shellfish toxins (PSTs) is often used as a model to study the migration, biotransformation,
accumulation, and removal of PSTs. However, the mechanism is still unclear. To provide a new tool
for related studies, we tried to label PSTs metabolically with 15N stable isotope to obtain 15N-PSTs
instead of original 14N, which could be treated as biomarker on PSTs metabolism. We then cultured
the A. minutum AGY-H46 which produces toxins GTX1-4 in f/2 medium of different 15N/P
concentrations. The 15N-PSTs’ toxicity and toxin profile were detected. Meanwhile, the 15N labeling
abundance and 15N atom number of 15N-PSTs were identified. The 14N of PSTs produced by A.
minutum can be successfully replaced by 15N, and the f/2 medium of standard 15N/P concentration
was the best choice in terms of the species’ growth, PST profile, 15N labeling result and experiment
cost. After many (>15) generations, the 15N abundance in PSTs extract reached 82.36%, and the 15N
atom number introduced into GTX1-4 might be 4–6. This paper innovatively provided the initial
evidence that 15N isotope application of labeling PSTs in A. minutum is feasible. The 15N-PSTs as
biomarker can be applied and provide further information on PSTs metabolism.
Share & Cite This Article
Xie, W.; Li, M.; Song, L.; Zhang, R.; Hu, X.; Liang, C.; Yang, X. 15N Stable Isotope Labeling PSTs in Alexandrium minutum for Application of PSTs as Biomarker. Toxins 2019, 11, 211.
Xie W, Li M, Song L, Zhang R, Hu X, Liang C, Yang X. 15N Stable Isotope Labeling PSTs in Alexandrium minutum for Application of PSTs as Biomarker. Toxins. 2019; 11(4):211.Chicago/Turabian Style
Xie, Wancui; Li, Min; Song, Lin; Zhang, Rui; Hu, Xiaoqun; Liang, Chengzhu; Yang, Xihong. 2019. "15N Stable Isotope Labeling PSTs in Alexandrium minutum for Application of PSTs as Biomarker." Toxins 11, no. 4: 211.
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