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Toxins 2019, 11(3), 164;

Regulation of Microcystin-LR-Induced DNA Damage by miR-451a in HL7702 Cells

Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha 410078, China
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University, Nanjing 210007, China
Key laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Central South University, Changsha 410083, China
Author to whom correspondence should be addressed.
Received: 21 February 2019 / Revised: 11 March 2019 / Accepted: 12 March 2019 / Published: 15 March 2019
(This article belongs to the Collection Toxicological Challenges of Aquatic Toxins)
PDF [1724 KB, uploaded 15 March 2019]


Microcystin-LR is a cyclic heptapeptide hepatotoxin produced by harmful cyanobacteria. A panel of microRNAs containing miR-451a were found to be significantly changed in normal human liver cells HL7702 after exposure to microcystin-LR (MC-LR) in our previous study. However, the functions of miR-451a in hepatotoxicity induced by MC-LR remained unclear. The study aimed to investigate the impacts of miR-451a in HL7702 cells following treatment with 5 or 10 μM MC-LR. The comet assay indicated that MC-LR can influence Olive tail moment (OTM) in HL7702 cells. Furthermore, increase of miR-451a significantly repressed DNA damage and the protein expression level of γ-H2AX induced by MC-LR. Moreover, over-expression of miR-451a inhibited the expression level of p-AKT1 protein in cells following treatment by MC-LR. These results showed that miR-451a may protect from MC-LR-induced DNA damage by down-regulating the expression of p-AKT1, which provides new clues for the diagnosis and therapy policies for liver damage induced by MC-LR. View Full-Text
Keywords: microcystin-LR; miR-451a; DNA damage; AKT1 microcystin-LR; miR-451a; DNA damage; AKT1

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Chen, L.; Yang, S.; Wen, C.; Zheng, S.; Yang, Y.; Feng, X.; Chen, J.; Luo, D.; Liu, R.; Yang, F. Regulation of Microcystin-LR-Induced DNA Damage by miR-451a in HL7702 Cells. Toxins 2019, 11, 164.

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