Effects of Essential Oil Citral on the Growth, Mycotoxin Biosynthesis and Transcriptomic Profile of Alternaria alternata
Abstract
:1. Introduction
2. Results
2.1. Inhibitory Effects on Mycelial Growth, Spore Germination and Mycotoxin Production
2.2. Global Analysis of Transcriptomic Profile
2.3. Functional Analysis of DEGs
2.4. Genes Responsible for Cell Integrity
2.5. Genes Related to Stress Response
2.6. Citral Interferes with the Expression of Genes Responsible for Mycotoxin Biosynthesis
3. Discussion
4. Materials and Methods
4.1. Chemicals, Strain and Culture Conditions
4.2. Antifungal Effects of Citral on Mycelial Growth and Spore Germination
4.3. Determination of Mycotoxin Production
4.4. Transcriptome Analysis
4.5. Detection of Fungal Ergosterol Content
4.6. Release of Intracellular Protein
4.7. Scanning Electron Microscopy Analysis
4.8. Analysis of Total Antioxidant Capacity and Catalase Activity
4.9. Transcriptional Validation of Biosynthetic Genes Involved in Mycotoxin Production
4.10. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Wang, L.; Jiang, N.; Wang, D.; Wang, M. Effects of Essential Oil Citral on the Growth, Mycotoxin Biosynthesis and Transcriptomic Profile of Alternaria alternata. Toxins 2019, 11, 553. https://doi.org/10.3390/toxins11100553
Wang L, Jiang N, Wang D, Wang M. Effects of Essential Oil Citral on the Growth, Mycotoxin Biosynthesis and Transcriptomic Profile of Alternaria alternata. Toxins. 2019; 11(10):553. https://doi.org/10.3390/toxins11100553
Chicago/Turabian StyleWang, Liuqing, Nan Jiang, Duo Wang, and Meng Wang. 2019. "Effects of Essential Oil Citral on the Growth, Mycotoxin Biosynthesis and Transcriptomic Profile of Alternaria alternata" Toxins 11, no. 10: 553. https://doi.org/10.3390/toxins11100553