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Open AccessArticle

Novel Polyclonal Antibody Raised against Tetrodotoxin Using Its Haptenic Antigen Prepared from 4,9-anhydrotetrodotoxin Reacted with 1,2-Ethaneditiol and Further Reacted with Keyhole Limpet Hemocyanin

School of Marine Biosciences, Kitasato University, Sagamihara-shi, Kanagawa 252-0373, Japan
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Author to whom correspondence should be addressed.
Toxins 2019, 11(10), 551; https://doi.org/10.3390/toxins11100551
Received: 30 July 2019 / Revised: 18 September 2019 / Accepted: 19 September 2019 / Published: 20 September 2019
(This article belongs to the Special Issue Marine Toxins Detection)
A novel polyclonal antibody against tetrodotoxin (TTX) was raised using its haptenic antigen, where 4,9-anhydroTTX was reacted with 1,2-ethanedithiol and this derivative was further reacted with keyhole limpet hemocyanin (KLH). This newly designed antigen (KLH-TTX) was inoculated into rabbits, resulting in the production of the specific polyclonal antibody, which reacted well with TTX and its analogs, 4-epiTTX, 11-oxoTTX and 5,6,11-trideoxyTTX, except for 4,9-anhydroTTX. The enzyme-linked immunosorbent assay (ELISA) system using this specific antibody was also developed in the present study. This newly developed polyclonal antibody with analytical procedures using direct one-step ELISA is useful to detect TTX and its analogs in toxic organisms and also disclose the mechanisms involved in their metabolic pathways and accumulation of TTX. View Full-Text
Keywords: tetrodotoxin; tetrodotoxin analogs; pufferfish; polyclonal antibody; ELISA tetrodotoxin; tetrodotoxin analogs; pufferfish; polyclonal antibody; ELISA
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Sato, S.; Takaishi, S.; Yasumoto, K.; Watabe, S. Novel Polyclonal Antibody Raised against Tetrodotoxin Using Its Haptenic Antigen Prepared from 4,9-anhydrotetrodotoxin Reacted with 1,2-Ethaneditiol and Further Reacted with Keyhole Limpet Hemocyanin. Toxins 2019, 11, 551.

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