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Volume 11
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Article

Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms

by
Lidia Riaño-Umbarila
1,
Ilse V. Gómez-Ramírez
2,
Luis M. Ledezma-Candanoza
2,
Timoteo Olamendi-Portugal
2,
Everardo Remi Rodríguez-Rodríguez
2,
Guillermo Fernández-Taboada
2,
Lourival D. Possani
2 and
Baltazar Becerril
2,*
1
CONACYT, Instituto de Biotecnología—Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
2
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, UNAM, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(1), 32; https://doi.org/10.3390/toxins11010032
Submission received: 11 December 2018 / Revised: 3 January 2019 / Accepted: 3 January 2019 / Published: 10 January 2019
(This article belongs to the Special Issue Discovery of Antibodies and Novel Antivenoms against Envenoming)
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Abstract

The recombinant antibody fragments generated against the toxic components of scorpion venoms are considered a promising alternative for obtaining new antivenoms for therapy. Using directed evolution and site-directed mutagenesis, it was possible to generate a human single-chain antibody fragment with a broad cross-reactivity that retained recognition for its original antigen. This variant is the first antibody fragment that neutralizes the effect of an estimated 13 neurotoxins present in the venom of nine species of Mexican scorpions. This single antibody fragment showed the properties of a polyvalent antivenom. These results represent a significant advance in the development of new antivenoms against scorpion stings, since the number of components would be minimized due to their broad cross-neutralization capacity, while at the same time bypassing animal immunization.
Keywords: cross-neutralization; directed evolution; phage display; polyvalent antivenom; scFv; scorpion cross-neutralization; directed evolution; phage display; polyvalent antivenom; scFv; scorpion
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MDPI and ACS Style

Riaño-Umbarila, L.; Gómez-Ramírez, I.V.; Ledezma-Candanoza, L.M.; Olamendi-Portugal, T.; Rodríguez-Rodríguez, E.R.; Fernández-Taboada, G.; Possani, L.D.; Becerril, B. Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms. Toxins 2019, 11, 32. https://doi.org/10.3390/toxins11010032

AMA Style

Riaño-Umbarila L, Gómez-Ramírez IV, Ledezma-Candanoza LM, Olamendi-Portugal T, Rodríguez-Rodríguez ER, Fernández-Taboada G, Possani LD, Becerril B. Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms. Toxins. 2019; 11(1):32. https://doi.org/10.3390/toxins11010032

Chicago/Turabian Style

Riaño-Umbarila, Lidia, Ilse V. Gómez-Ramírez, Luis M. Ledezma-Candanoza, Timoteo Olamendi-Portugal, Everardo Remi Rodríguez-Rodríguez, Guillermo Fernández-Taboada, Lourival D. Possani, and Baltazar Becerril. 2019. "Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms" Toxins 11, no. 1: 32. https://doi.org/10.3390/toxins11010032

APA Style

Riaño-Umbarila, L., Gómez-Ramírez, I. V., Ledezma-Candanoza, L. M., Olamendi-Portugal, T., Rodríguez-Rodríguez, E. R., Fernández-Taboada, G., Possani, L. D., & Becerril, B. (2019). Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms. Toxins, 11(1), 32. https://doi.org/10.3390/toxins11010032

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