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Toxins 2018, 10(9), 375; https://doi.org/10.3390/toxins10090375

Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS)

1
Laboratory for Food Safety, ANSES, Université Paris-Est, F-94701 Maisons-Alfort, France
2
Agreenium, the French Agricultural, Veterinary and Forestry Institute, 75116 Paris, France
*
Author to whom correspondence should be addressed.
Received: 11 July 2018 / Revised: 27 August 2018 / Accepted: 11 September 2018 / Published: 14 September 2018
(This article belongs to the Special Issue Emerging Marine Biotoxins)
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Abstract

When considering the geographical expansion of marine toxins, the emergence of new toxins and the associated risk for human health, there is urgent need for versatile and efficient analytical methods that are able to detect a range, as wide as possible, of known or emerging toxins. Current detection methods for marine toxins rely on a priori defined target lists of toxins and are generally inappropriate for the detection and identification of emerging compounds. The authors describe the implementation of a recent approach for the non-targeted analysis of marine toxins in shellfish with a focus on a comprehensive workflow for the acquisition and treatment of the data generated after liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) analysis. First, the study was carried out in targeted mode to assess the performance of the method for known toxins with an extended range of polarities, including lipophilic toxins (okadaic acid, dinophysistoxins, azaspiracids, pectenotoxins, yessotoxins, cyclic imines, brevetoxins) and domoic acid. The targeted method, assessed for 14 toxins, shows good performance both in mussel and oyster extracts. The non-target potential of the method was then challenged via suspects and without a priori screening by blind analyzing mussel and oyster samples spiked with marine toxins. The data processing was optimized and successfully identified the toxins that were spiked in the blind samples. View Full-Text
Keywords: marine toxins; LC-HRMS; targeted analysis; suspects screening; non-targeted analysis; method characterization marine toxins; LC-HRMS; targeted analysis; suspects screening; non-targeted analysis; method characterization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Dom, I.; Biré, R.; Hort, V.; Lavison-Bompard, G.; Nicolas, M.; Guérin, T. Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS). Toxins 2018, 10, 375.

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