Next Article in Journal
Deleting Death and Dialysis: Conservative Care of Cardio-Vascular Risk and Kidney Function Loss in Chronic Kidney Disease (CKD)
Next Article in Special Issue
Current Advances in Immunological Studies on the Vespidae Venom Antigen 5: Therapeutic and Prophylaxis to Hypersensitivity Responses
Previous Article in Journal
Resveratrol Ameliorates Microcystin-LR-Induced Testis Germ Cell Apoptosis in Rats via SIRT1 Signaling Pathway Activation
Previous Article in Special Issue
Antivenom Production against Bothrops jararaca and Bothrops erythromelas Snake Venoms Using Cross-Linked Chitosan Nanoparticles as an Immunoadjuvant

Basics of Antibody Phage Display Technology

Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby DK-2800, Denmark
IONTAS Ltd., Cambridgeshire CB22 3EG, United Kingdom
Author to whom correspondence should be addressed.
Toxins 2018, 10(6), 236;
Received: 31 May 2018 / Revised: 7 June 2018 / Accepted: 8 June 2018 / Published: 9 June 2018
(This article belongs to the Special Issue Discovery of Antibodies and Novel Antivenoms against Envenoming)
Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the M13 phage and the basics of phage display technology are presented together with important factors influencing the success rates of phage display experiments. Moreover, the pros and cons of different antigen display methods and the use of naïve versus immunized phage display antibody libraries is discussed, and selected examples from the field of antivenom research are highlighted. This review thus provides in-depth knowledge on the principles and use of phage display technology with a special focus on discovery of antibodies that target animal toxins. View Full-Text
Keywords: antibody discovery; recombinant antivenom; phage display; M13 phage; toxinology antibody discovery; recombinant antivenom; phage display; M13 phage; toxinology
Show Figures

Figure 1

MDPI and ACS Style

Ledsgaard, L.; Kilstrup, M.; Karatt-Vellatt, A.; McCafferty, J.; Laustsen, A.H. Basics of Antibody Phage Display Technology. Toxins 2018, 10, 236.

AMA Style

Ledsgaard L, Kilstrup M, Karatt-Vellatt A, McCafferty J, Laustsen AH. Basics of Antibody Phage Display Technology. Toxins. 2018; 10(6):236.

Chicago/Turabian Style

Ledsgaard, Line, Mogens Kilstrup, Aneesh Karatt-Vellatt, John McCafferty, and Andreas H. Laustsen. 2018. "Basics of Antibody Phage Display Technology" Toxins 10, no. 6: 236.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop