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Biomarker of Aflatoxin Ingestion: 1H NMR-Based Plasma Metabolomics of Dairy Cows Fed Aflatoxin B1 with or without Sequestering Agents

1
College of Agriculture, Communities, and the Environment, Kentucky State University, Frankfort, KY 40601, USA
2
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
3
Institute of Agriculture and Environmental Sciences, Federal University of Mato Grosso, Sinop, MT 78557-267, Brazil
*
Author to whom correspondence should be addressed.
Toxins 2018, 10(12), 545; https://doi.org/10.3390/toxins10120545
Received: 15 November 2018 / Revised: 7 December 2018 / Accepted: 11 December 2018 / Published: 18 December 2018
(This article belongs to the Special Issue Metabolomics in Mycotoxin Research)
The study applied 1H NMR-based plasma metabolomics to identify candidate biomarkers of aflatoxin B1 (AFB1) ingestion in dairy cows fed no sequestering agents and evaluate the effect of supplementing clay and/or a Saccharomyces cerevisiae fermentation product (SCFP) on such biomarkers. Eight lactating cows were randomly assigned to 1 of 4 treatments in a balanced 4 × 4 Latin square design with 2 squares. Treatments were: control, toxin (T; 1725 µg AFB1/head/day), T with clay (CL; 200 g/head/day), and CL with SCFP (CL + SCFP; 35 g of SCFP/head/day). Cows in T, CL, and CL + SCFP were dosed with AFB1 from d 26 to 30. The sequestering agents were top-dressed from d 1 to 33. On d 30 of each period, 15 mL of blood was taken from the coccygeal vessels and plasma samples were prepared by centrifugation. Compared to the control, T decreased plasma concentrations of alanine, acetic acid, leucine, arginine and valine. In contrast, T increased plasma ethanol concentration 3.56-fold compared to control. Treatment with CL tended to reduce sarcosine concentration, whereas treatment with CL + SCFP increased concentrations of mannose and 12 amino acids. Based on size of the area under the curve (AUC) of receiver operating characteristic and fold change (FC) analyses, ethanol was the most significantly altered metabolite in T (AUC = 0.88; FC = 3.56); hence, it was chosen as the candidate biomarker of aflatoxin ingestion in dairy cows fed no sequestering agent. View Full-Text
Keywords: aflatoxin; dairy cow; metabolomics; plasma aflatoxin; dairy cow; metabolomics; plasma
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MDPI and ACS Style

Ogunade, I.; Jiang, Y.; Adeyemi, J.; Oliveira, A.; Vyas, D.; Adesogan, A. Biomarker of Aflatoxin Ingestion: 1H NMR-Based Plasma Metabolomics of Dairy Cows Fed Aflatoxin B1 with or without Sequestering Agents. Toxins 2018, 10, 545. https://doi.org/10.3390/toxins10120545

AMA Style

Ogunade I, Jiang Y, Adeyemi J, Oliveira A, Vyas D, Adesogan A. Biomarker of Aflatoxin Ingestion: 1H NMR-Based Plasma Metabolomics of Dairy Cows Fed Aflatoxin B1 with or without Sequestering Agents. Toxins. 2018; 10(12):545. https://doi.org/10.3390/toxins10120545

Chicago/Turabian Style

Ogunade, Ibukun, Yun Jiang, James Adeyemi, Andre Oliveira, Diwakar Vyas, and Adegbola Adesogan. 2018. "Biomarker of Aflatoxin Ingestion: 1H NMR-Based Plasma Metabolomics of Dairy Cows Fed Aflatoxin B1 with or without Sequestering Agents" Toxins 10, no. 12: 545. https://doi.org/10.3390/toxins10120545

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