Fibroblast Growth Factor Receptor, a Novel Receptor for Vegetative Insecticidal Protein Vip3Aa
1
Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China
2
State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China
3
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, China
4
Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin 300071, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Toxins 2018, 10(12), 546; https://doi.org/10.3390/toxins10120546
Received: 27 November 2018
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Revised: 12 December 2018
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Accepted: 14 December 2018
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Published: 18 December 2018
(This article belongs to the Special Issue Insecticidal Toxins from Bacillus thuringiensis)
Vegetative insecticidal proteins (Vips), which are secreted by some Bacillus thuringiensis strains during vegetative growth, exhibit high virulence to many pests. Vip3A proteins have been used commercially both in some bio-insecticides and in transgenic crops; however, compared with insecticidal crystal proteins, the mechanism of action of Vip3A is still unclear. In this work, we indicated that the fibroblast growth factor receptor-like protein (Sf-FGFR) from the membrane of Sf9 cells could bind to Vip3Aa. The interaction between Vip3Aa and Sf-FGFR was confirmed by pull-down assays and dot blotting experiment in vitro. The binding affinity between Vip3Aa and extracellular regions of Sf-FGFR (GST-FGFR-N) was determined by microscale thermophoresis assay (MST). Moreover, Vip3Aa-Flag could be co-immunoprecipitated with Sf-FGFR-V5 ex vivo. Furthermore, knockdown of Sf-FGFR gene in Sf9 cells resulted in reducing the mortality of those cells to Vip3Aa. In summary, our data indicated that Sf-FGFR is a novel receptor for Vip3Aa.
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MDPI and ACS Style
Jiang, K.; Hou, X.; Han, L.; Tan, T.; Cao, Z.; Cai, J. Fibroblast Growth Factor Receptor, a Novel Receptor for Vegetative Insecticidal Protein Vip3Aa. Toxins 2018, 10, 546. https://doi.org/10.3390/toxins10120546
AMA Style
Jiang K, Hou X, Han L, Tan T, Cao Z, Cai J. Fibroblast Growth Factor Receptor, a Novel Receptor for Vegetative Insecticidal Protein Vip3Aa. Toxins. 2018; 10(12):546. https://doi.org/10.3390/toxins10120546
Chicago/Turabian StyleJiang, Kun, Xiaoyue Hou, Lu Han, Tongtong Tan, Zhanglei Cao, and Jun Cai. 2018. "Fibroblast Growth Factor Receptor, a Novel Receptor for Vegetative Insecticidal Protein Vip3Aa" Toxins 10, no. 12: 546. https://doi.org/10.3390/toxins10120546
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