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Urinary Metabolomic Approach Provides New Insights into Distinct Metabolic Profiles of Glutamine and N-Carbamylglutamate Supplementation in Rats

by Guangmang Liu 1,2,*, Wei Cao 1,2, Tingting Fang 1,2, Gang Jia 1,2, Hua Zhao 1,2, Xiaoling Chen 1,2, Caimei Wu 1,2 and Jing Wang 3
1
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
2
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
3
Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
*
Author to whom correspondence should be addressed.
Nutrients 2016, 8(8), 478; https://doi.org/10.3390/nu8080478
Received: 8 April 2016 / Revised: 13 July 2016 / Accepted: 28 July 2016 / Published: 4 August 2016
Glutamine and N-carbamylglutamate can enhance growth performance and health in animals, but the underlying mechanisms are not yet elucidated. This study aimed to investigate the effect of glutamine and N-carbamylglutamate supplementation in rat metabolism. Thirty rats were fed a control, glutamine, or N-carbamylglutamate diet for four weeks. Urine samples were analyzed by nuclear magnetic resonance (NMR)-based metabolomics, specifically high-resolution 1H NMR metabolic profiling combined with multivariate data analysis. Glutamine significantly increased the urine levels of acetamide, acetate, citrulline, creatinine, and methymalonate, and decreased the urine levels of ethanol and formate (p < 0.05). Moreover, N-carbamylglutamate significantly increased the urine levels of creatinine, ethanol, indoxyl sulfate, lactate, methymalonate, acetoacetate, m-hydroxyphenylacetate, and sarcosine, and decreased the urine levels of acetamide, acetate, citrulline, creatine, glycine, hippurate, homogentisate, N-acetylglutamate, phenylacetyglycine, acetone, and p-hydroxyphenylacetate (p < 0.05). Results suggested that glutamine and N-carbamylglutamate could modify urinary metabolome related to nitrogen metabolism and gut microbiota metabolism. Moreover, N-carbamylglutamate could alter energy and lipid metabolism. These findings indicate that different arginine precursors may lead to differences in the biofluid profile in rats. View Full-Text
Keywords: glutamine; N-carbamylglutamate; metabolism; metabolomics; urine glutamine; N-carbamylglutamate; metabolism; metabolomics; urine
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Liu, G.; Cao, W.; Fang, T.; Jia, G.; Zhao, H.; Chen, X.; Wu, C.; Wang, J. Urinary Metabolomic Approach Provides New Insights into Distinct Metabolic Profiles of Glutamine and N-Carbamylglutamate Supplementation in Rats. Nutrients 2016, 8, 478.

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