Salvianolic Acid B Inhibits Aβ Generation by Modulating BACE1 Activity in SH-SY5Y-APPsw Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Drugs, Reagents and Antibodies
2.2. Cell Culture
2.3. ELISA
2.4. Reactive Oxygen Species (ROS) Production
2.5. MDA, SOD and GSH-Px Assays
2.6. Western Blot Assay
2.7. Statistical Analysis
3. Results
3.1. SalB Reduces the Levels of Aβ40 and Aβ42 in Cell Culture Medium
3.2. SalB Reduces the Level of sAPPβ in SH-SY5Y-APPsw Cells
3.3. SalB Decreases the Protein Expression and the Activity of BACE1 in SH-SY5Y-APPsw Cells
3.4. SalB Ameliorates Oxidative Stress and Inhibits GSK3β Activity in SH-SY5Y-APPsw Cells
4. Discussion
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Tang, Y.; Huang, D.; Zhang, M.-H.; Zhang, W.-S.; Tang, Y.-X.; Shi, Z.-X.; Deng, L.; Zhou, D.-H.; Lu, X.-Y. Salvianolic Acid B Inhibits Aβ Generation by Modulating BACE1 Activity in SH-SY5Y-APPsw Cells. Nutrients 2016, 8, 333. https://doi.org/10.3390/nu8060333
Tang Y, Huang D, Zhang M-H, Zhang W-S, Tang Y-X, Shi Z-X, Deng L, Zhou D-H, Lu X-Y. Salvianolic Acid B Inhibits Aβ Generation by Modulating BACE1 Activity in SH-SY5Y-APPsw Cells. Nutrients. 2016; 8(6):333. https://doi.org/10.3390/nu8060333
Chicago/Turabian StyleTang, Ying, Dan Huang, Mei-Hua Zhang, Wen-Sheng Zhang, Yu-Xin Tang, Zheng-Xiang Shi, Li Deng, Dai-Han Zhou, and Xin-Yi Lu. 2016. "Salvianolic Acid B Inhibits Aβ Generation by Modulating BACE1 Activity in SH-SY5Y-APPsw Cells" Nutrients 8, no. 6: 333. https://doi.org/10.3390/nu8060333