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Open AccessArticle

1H NMR Fingerprinting of Soybean Extracts, with Emphasis on Identification and Quantification of Isoflavones

Dipartimento di Chimica Organica e Industriale, Università di Parma, Via Usberti 17A, 43100-Parma, Italy
Dipartimento di Scienze Farmaceutiche, Università di Ferrara, Via Fossato di Mortara 17/19, 44100 Ferrara, Italy
Author to whom correspondence should be addressed.
Nutrients 2010, 2(3), 280-289;
Received: 17 December 2009 / Accepted: 24 February 2010 / Published: 26 February 2010
(This article belongs to the Special Issue Foodomics 2009)
PDF [287 KB, uploaded 26 February 2010]


1H NMR spectra were recorded of methanolic extracts of seven soybean varieties (Glycine max.), cultivated using traditional and organic farming techniques. It was possible to identify signals belonging to the groups of amino acids, carbohydrates, organic acids and aromatic substances in the spectra. In the aromatic zone, the isoflavone signals were of particular interest: genistein, daidzein, genistin, daidzin, malonylgenistin, acetylgenistin, malonyldaidzin signals were assigned and these compounds were quantified, resulting in accordance with published data, and further demonstrating the potential of the NMR technique in food science. View Full-Text
Keywords: 1H NMR; soybean; isoflavones; fingerprinting 1H NMR; soybean; isoflavones; fingerprinting

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Caligiani, A.; Palla, G.; Maietti, A.; Cirlini, M.; Brandolini, V. 1H NMR Fingerprinting of Soybean Extracts, with Emphasis on Identification and Quantification of Isoflavones. Nutrients 2010, 2, 280-289.

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