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Volume 16
Issue 19
10.3390/nu16193418
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Article

Transport of Neutral Amino Acids in the Jejunum of Pigs with Special Consideration of L-Methionine

by
Isabel I. Schermuly
1,
Stella Romanet
1,
Amlan K. Patra
2,
Lucia Mastrototaro
1,3,
Andreas Lemme
4,
Robert Pieper
5,
Jürgen Zentek
5 and
Jörg R. Aschenbach
1,*
1
Institute of Veterinary Physiology, Freie Universität Berlin, Königsweg 56, 14163 Berlin, Germany
2
American Institute for Goat Research, Langston University, Langston, OK 73050, USA
3
Institute for Clinical Diabetology, German Diabetes Center, Leibniz Institute for Diabetes Research at Heinrich-Heine-University, Auf’m Hennekamp 65, 40225 Düsseldorf, Germany
4
Animal Nutrition Services, Evonik Operations GmbH, Rodenbacher Chausee 4, 63457 Hanau-Wolfgang, Germany
5
Institute of Animal Nutrition, Freie Universität Berlin, Königin-Luise-Straße 49, 14195 Berlin, Germany
*
Author to whom correspondence should be addressed.
Nutrients 2024, 16(19), 3418; https://doi.org/10.3390/nu16193418
Submission received: 20 September 2024 / Revised: 5 October 2024 / Accepted: 7 October 2024 / Published: 9 October 2024
(This article belongs to the Section Proteins and Amino Acids)
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Abstract

Background: Methionine (Met) is a popular nutritional supplement in humans and animals. It is routinely supplemented to pigs as L-Met, DL-Met, or DL-2-hydroxy-4-(methylthio) butanoic acid (DL-HMTBA). Methods: We investigated the effect of these Met supplements on jejunal amino acid (AA) transport in male castrated Piétrain × Danbred pigs, also including a non-supplemented group. The mucosal-to-serosal flux of ten [14C]-labeled AAs (L-glutamine, glycine, L-leucine, L-lysine, L-Met, L-serine, L-threonine, L-tryptophan, L-tyrosine and L-valine) was investigated at two concentrations (50 µM and 5 mM). Inhibition of apical uptake by mucosal L-Met was also measured for these AAs. The intestinal expression of apical AA transporters, angiotensin-converting enzyme II and inflammation-related genes were compared with those of a previous study. Results: Except for tryptophan and lysine at 5 mM, all AA fluxes were Na+-dependent (p ≤ 0.05), and the uptake of most AAs, except glycine and lysine, was inhibited by L-Met (p < 0.001). A correlation network existed between Na+-dependent fluxes of most AAs (except tryptophan and partly glycine). We observed the upregulation of B0AT1 (SLC6A19) (p < 0.001), the downregulation of ATB0,+ (SLC6A14) (p < 0.001) and a lower expression of CASP1, IL1β, IL8, TGFβ and TNFα in the present vs. the previous study (p < 0.001). Conclusions: The correlating AAs likely share the same Na+-dependent transporter(s). A varying effect of the Met supplement type on AA transport in the two studies might be related to a different level of supplementation or a different inflammatory status of the small intestine.
Keywords: amino acid interaction; methionine supplementation; piglets; intestinal absorption; intestinal inflammation; Ussing chamber; angiotensin-converting enzyme II amino acid interaction; methionine supplementation; piglets; intestinal absorption; intestinal inflammation; Ussing chamber; angiotensin-converting enzyme II

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MDPI and ACS Style

Schermuly, I.I.; Romanet, S.; Patra, A.K.; Mastrototaro, L.; Lemme, A.; Pieper, R.; Zentek, J.; Aschenbach, J.R. Transport of Neutral Amino Acids in the Jejunum of Pigs with Special Consideration of L-Methionine. Nutrients 2024, 16, 3418. https://doi.org/10.3390/nu16193418

AMA Style

Schermuly II, Romanet S, Patra AK, Mastrototaro L, Lemme A, Pieper R, Zentek J, Aschenbach JR. Transport of Neutral Amino Acids in the Jejunum of Pigs with Special Consideration of L-Methionine. Nutrients. 2024; 16(19):3418. https://doi.org/10.3390/nu16193418

Chicago/Turabian Style

Schermuly, Isabel I., Stella Romanet, Amlan K. Patra, Lucia Mastrototaro, Andreas Lemme, Robert Pieper, Jürgen Zentek, and Jörg R. Aschenbach. 2024. "Transport of Neutral Amino Acids in the Jejunum of Pigs with Special Consideration of L-Methionine" Nutrients 16, no. 19: 3418. https://doi.org/10.3390/nu16193418

APA Style

Schermuly, I. I., Romanet, S., Patra, A. K., Mastrototaro, L., Lemme, A., Pieper, R., Zentek, J., & Aschenbach, J. R. (2024). Transport of Neutral Amino Acids in the Jejunum of Pigs with Special Consideration of L-Methionine. Nutrients, 16(19), 3418. https://doi.org/10.3390/nu16193418

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