β-Hydroxy-β-methyl Butyrate Regulates the Lipid Metabolism, Mitochondrial Function, and Fat Browning of Adipocytes
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture
2.3. Cell Proliferation Analysis
2.4. Oil Red O Staining
2.5. The Determination of TG Levels
2.6. Western Blotting Analysis
2.7. Enzyme-Linked Immunosorbent Assay (ELISA)
2.8. Fatty Acid Composition Analysis
2.9. Seahorse Metabolic Assay
2.10. Statistical Analysis
3. Results
3.1. HMB Suppressed Lipid Accumulation in 3T3-L1 Adipocytes
3.2. HMB Altered the Concentrations of Lipid Metabolism-Related Enzymes and Fatty Acid Composition in 3T3-L1 Adipocytes
3.3. HMB Improved the Mitochondrial Respiratory Function of 3T3-L1 Adipocytes
3.4. HMB Promoted the Browning of 3T3-L1 Adipocytes
4. Discussion
5. Conclusions
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Item | Control | HMB | SEM | p-Value |
---|---|---|---|---|
C10:0 | 0.61 b | 0.90 a | 0.00076 | <0.05 |
C14:0 | 3.71 a | 2.68 b | 0.00254 | <0.05 |
C15:0 | 8.12 a | 5.45 b | 0.00655 | <0.05 |
C16:0 | 31.71 | 35.24 | 0.01101 | 0.11 |
C16:1 | 21.38 a | 12.60 b | 0.02117 | <0.01 |
C17:0 | 2.41 a | 2.06 b | 0.00090 | <0.05 |
C18:0 | 12.31 b | 21.28 a | 0.02119 | <0.01 |
C18:1n9c | 13.06 | 11.96 | 0.00739 | 0.55 |
C22:1n9 | 2.53 | 4.10 | 0.00502 | 0.12 |
C20:4n6 | 1.56 b | 2.15 a | 0.00132 | <0.001 |
C22:6n3 | 0.68 b | 0.93 a | 0.00057 | <0.001 |
∑n6 PUFAs | 1.56 b | 2.15 a | 0.00132 | <0.001 |
∑n3 PUFAs | 0.68 b | 0.93 a | 0.00057 | <0.001 |
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Duan, G.; Zheng, C.; Yu, J.; Zhang, P.; Wan, M.; Zheng, J.; Duan, Y. β-Hydroxy-β-methyl Butyrate Regulates the Lipid Metabolism, Mitochondrial Function, and Fat Browning of Adipocytes. Nutrients 2023, 15, 2550. https://doi.org/10.3390/nu15112550
Duan G, Zheng C, Yu J, Zhang P, Wan M, Zheng J, Duan Y. β-Hydroxy-β-methyl Butyrate Regulates the Lipid Metabolism, Mitochondrial Function, and Fat Browning of Adipocytes. Nutrients. 2023; 15(11):2550. https://doi.org/10.3390/nu15112550
Chicago/Turabian StyleDuan, Geyan, Changbing Zheng, Jiayi Yu, Peiwen Zhang, Mengliao Wan, Jie Zheng, and Yehui Duan. 2023. "β-Hydroxy-β-methyl Butyrate Regulates the Lipid Metabolism, Mitochondrial Function, and Fat Browning of Adipocytes" Nutrients 15, no. 11: 2550. https://doi.org/10.3390/nu15112550
APA StyleDuan, G., Zheng, C., Yu, J., Zhang, P., Wan, M., Zheng, J., & Duan, Y. (2023). β-Hydroxy-β-methyl Butyrate Regulates the Lipid Metabolism, Mitochondrial Function, and Fat Browning of Adipocytes. Nutrients, 15(11), 2550. https://doi.org/10.3390/nu15112550