Vitamin A Ameliorated Irinotecan-Induced Diarrhea in a Piglet Model Involving Enteric Glia Modulation and Immune Cells Infiltration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Chemicals
2.2. Animals and CPT-Induced Experimental Diarrhea of Piglets
2.3. Cell Culture and Treatment
2.4. Morphological Examination
2.5. RNA Extraction and Quantitative Real-Time Polymerase Chain Reaction
2.6. Western Blot (WB) Analysis and Enzyme-Linked Immunosorbent Assay (ELISA)
2.7. Immunofluorescence
2.8. Statistical Analysis
3. Results
3.1. Dietary VA Reduced Diarrhea Incidence and Attenuated Intestinal Damage Induced by CPT-11
3.2. VA Suppresses Immune Cell Infiltration and Inflammatory Responses
3.3. VA Inhibits Reactive Enteric Gliosis and Modulates Neuropeptides Production Following CPT-11 Challenge
3.4. RA Regulates the Functions of Macrophages and EGCs In Vitro
3.5. GDNF Modulates Macrophage Functions In Vitro
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Items | Primer Sequence (5′–3′) | Accession Number | Size (bp) |
---|---|---|---|
Rattus norvegicus β-actin | F: GGCTGTATTCCCCTCCATCG R: CCAGTTGGTAACAATGCCATGT | NM_001083538.3 | 154 |
Rattus norvegicus Gapdh | F: TGGCAAAGTGGAGATTGTTGCC R: CCAGTTGGTAACAATGCCATGT | XM_017005081.2 | 33 |
Rattus norvegicus Gdnf | F: TCCAACTGGGGGTCTACGG R: GCCACGACATCCCATAACTTCAT | NM_001301332.1 | 82 |
Rattus norvegicus Bdnf | F: TCATACTTCGGTTGCATGAAGG R: ACACCTGGGTAGGCCAAGTT | XM_051183802.1 | 102 |
Rattus norvegicus Il1b | F: CAGAAGTACCTGAGCTCGCC R: AGATTCGTAGCTGGATGCCG | NM_000576.3 | 153 |
Mouse β-actin | F: GGCTGTATTCCCCTCCATCG R: CCAGTTGGTAACAATGCCATGT | NM_001083538.3 | 154 |
Mouse Gapdh | F: TGGCAAAGTGGAGATTGTTGCC R: AAGATGGTGATGGGCTTCCCG | NM_001411843.1 | 114 |
Mouse Tnfa | F: CCCTCACACTCAGATCATCTTCT R: GCTACGACGTGGGCTACAG | NM_000594.4 | 61 |
Mouse Il1b | F: GCAACTGTTCCTGAACTCAACT R: ATCTTTTGGGGTCCGTCAACT | NM_008361.4 | 89 |
Mouse Il6 | F: CTACTGCCTTCCCTACCC R: ACCTCCTTGCTGTTTTCA | NM_001289394.2 | 102 |
Mouse Inos | F: AAGTCAAATCCTACCAAAGTGA R: CCATAATACTGGTTGATGAACT | NM_010927.4 | 409 |
Mouse Cox2 | F: GGGTGTGAAGGGAAATAAGG R: TCTCCACCAATGACCTGAT | NM_011198.4 | 291 |
Sus sucrofa Actin | F: CCACGAAACTACCTTCAACTCC R: GTGATCTCCTTCTGCATCCTGT | XM_021086047.1 | 132 |
Sus sucrofa apdh | F: AGAACATCATCCCAGCGTCC R: CGGCAGGTCAGGTCAACAAC | XM_021097231.1 | 133 |
Sus sucrofa Saa | F: AGCTTTCCACGGGCATCATT R: TCTTTAGCCCCTTGACCAGC | XM_013994503.2 | 97 |
Sus sucrofa Rara | F: GCATCCAGAAGAACATGGTGT R: CCTGCTTGGCGAACTCCACAGT | XM_003131473.4 | 393 |
Sus sucrofa Cebpα | F: GGTGGACAAGAACAGCAACG R: AGGCACCGGAATCTCCTAGT | XM_003127015.4 | 369 |
Sus sucrofa Cebpβ | F: GCTTGAACAAGTTCCGCAGG R: CAAGAAGACGGTGGACAAGC | NM_001199889.1 | 209 |
Sus sucrofa Il6 | F: GGAGACCTGCTTGATGAGAATC R: GTACTAATCTGCACAGCCTCGAC | NM_214399.1 | 117 |
Sus sucrofa Il1b | F: GAGCTGAAGGCTCTCCACCTC R: ATCGCTGTCATCTCCTTGCAC | NM_001302388.2 | 87 |
Sus sucrofa Il17 | F: CCAGACGGCCCTCAGATTAC R: CACTTGGCCTCCCAGATCAC | NM_001005729.1 | 103 |
Sus sucrofa Cox2 | F: GTGTGAAAGGGAGGAAAGA R: AAACTGATGGGTGAAGTGC | NM_214321.1 | 137 |
Sus sucrofa p65 | F: GTGTGTAAAGAAGCGGGACCT R: CACTGTCACCTGGAAGCAGAG | NM_001114281.1 | 139 |
Sus sucrofa p38 | F: CTTACGGATGACCCACGTTCAGT R: GCTCACAGTCTTCATTCACAGC | XM_013977842.2 | 128 |
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Li, M.; Huang, Y.; Jin, H.; Yuan, D.; Huang, K.; Wang, J.; Tan, B.; Yin, Y. Vitamin A Ameliorated Irinotecan-Induced Diarrhea in a Piglet Model Involving Enteric Glia Modulation and Immune Cells Infiltration. Nutrients 2022, 14, 5120. https://doi.org/10.3390/nu14235120
Li M, Huang Y, Jin H, Yuan D, Huang K, Wang J, Tan B, Yin Y. Vitamin A Ameliorated Irinotecan-Induced Diarrhea in a Piglet Model Involving Enteric Glia Modulation and Immune Cells Infiltration. Nutrients. 2022; 14(23):5120. https://doi.org/10.3390/nu14235120
Chicago/Turabian StyleLi, Meng, Yonggang Huang, Huimin Jin, Daixiu Yuan, Ke Huang, Jing Wang, Bie Tan, and Yulong Yin. 2022. "Vitamin A Ameliorated Irinotecan-Induced Diarrhea in a Piglet Model Involving Enteric Glia Modulation and Immune Cells Infiltration" Nutrients 14, no. 23: 5120. https://doi.org/10.3390/nu14235120