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Nutrients
Volume 14
Issue 23
10.3390/nu14235120
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Article

Vitamin A Ameliorated Irinotecan-Induced Diarrhea in a Piglet Model Involving Enteric Glia Modulation and Immune Cells Infiltration

by
Meng Li
1,2,3,
Yonggang Huang
1,
Huimin Jin
1,
Daixiu Yuan
4,
Ke Huang
1,
Jing Wang
1,
Bie Tan
1,2,* and
Yulong Yin
1,2
1
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
2
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
3
Hainan Institute, Zhejiang University, Sanya 572000, China
4
Department of Medicine, Jishou University, Jishou 416000, China
*
Author to whom correspondence should be addressed.
Nutrients 2022, 14(23), 5120; https://doi.org/10.3390/nu14235120
Submission received: 11 September 2022 / Revised: 25 November 2022 / Accepted: 26 November 2022 / Published: 2 December 2022
(This article belongs to the Special Issue Vitamins, Nutritional Supplements, Antioxidant Therapy, and Modulators of Inflammation as Therapeutic Interventions)
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Abstract

Vitamin A (VA) and its metabolite, retinoic acid (RA), play important roles in modulating intestinal mucosal immunity, yet little is known about their regulatory effects on enteric nervous system function. The study aims to explore the protective effects of dietary VA on diarrhea in a piglet model involving enteric glia and immune cell modulation. Twenty-eight weaned piglets were fed either the basal or VA (basal diet supplemented with 18,000 IU/kg VA) diet and with or without irinotecan (CPT-11) injection. CPT-11 induced increased diarrhea incidence, immune infiltration, and reactive enteric gliosis. A diet supplemented with 18,000 IU/kg VA ameliorated the adverse effects of CPT-11 on the gut barrier. VA reduced diarrhea incidence and attenuated enteric glial gliosis, immune cell infiltrations, and inflammatory responses of CPT-induced piglets. An in vitro experiment with 1 nmol/L RA showed direct protective effects on monocultures of enteric glial cells (EGCs) or macrophages in LPS-simulated inflammatory conditions. Furthermore, 1 ng/mL glial-derived neurotropic factors (GDNF) could inhibit M1-macrophage polarization and pro-inflammatory cytokines production. In summary, VA exerted protective effects on the intestinal barrier by modulating enteric glia and immune cells, perhaps enhancing epithelial recovery under CPT-11 challenge. Our study demonstrated that RA signaling might promote the roles of enteric glia in intestinal immunity and tissue repair, which provided a reference for the VA supplementation of patient diets.
Keywords: vitamin A; retinoic acid signaling; diarrhea; enteric glia; macrophage vitamin A; retinoic acid signaling; diarrhea; enteric glia; macrophage

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MDPI and ACS Style

Li, M.; Huang, Y.; Jin, H.; Yuan, D.; Huang, K.; Wang, J.; Tan, B.; Yin, Y. Vitamin A Ameliorated Irinotecan-Induced Diarrhea in a Piglet Model Involving Enteric Glia Modulation and Immune Cells Infiltration. Nutrients 2022, 14, 5120. https://doi.org/10.3390/nu14235120

AMA Style

Li M, Huang Y, Jin H, Yuan D, Huang K, Wang J, Tan B, Yin Y. Vitamin A Ameliorated Irinotecan-Induced Diarrhea in a Piglet Model Involving Enteric Glia Modulation and Immune Cells Infiltration. Nutrients. 2022; 14(23):5120. https://doi.org/10.3390/nu14235120

Chicago/Turabian Style

Li, Meng, Yonggang Huang, Huimin Jin, Daixiu Yuan, Ke Huang, Jing Wang, Bie Tan, and Yulong Yin. 2022. "Vitamin A Ameliorated Irinotecan-Induced Diarrhea in a Piglet Model Involving Enteric Glia Modulation and Immune Cells Infiltration" Nutrients 14, no. 23: 5120. https://doi.org/10.3390/nu14235120

APA Style

Li, M., Huang, Y., Jin, H., Yuan, D., Huang, K., Wang, J., Tan, B., & Yin, Y. (2022). Vitamin A Ameliorated Irinotecan-Induced Diarrhea in a Piglet Model Involving Enteric Glia Modulation and Immune Cells Infiltration. Nutrients, 14(23), 5120. https://doi.org/10.3390/nu14235120

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