Is It Possible to Improve the Bioavailability of Resveratrol and Polydatin Derived from Polygoni cuspidati Radix as a Result of Preparing Electrospun Nanofibers Based on Polyvinylpyrrolidone/Cyclodextrin?
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Chemicals and Reagents
2.3. Obtaining and Charakteristion of Biological Activity of Polygoni Cuspidati Extract
2.3.1. Plant Extraction Using Design of Experiment (DoE)
2.3.2. Determination of Selected Active Components Content and Total Phenolic Content (TPC)
2.3.3. Determination of Biological Activity
Antioxidant Activity
Anti-Hyaluronidase Activity
2.4. Obtaining of Electrospun Nanofibers Containing Polygoni Cuspidati Extract
Electrospun Nanofibers’ Preparation Using Design of Experiment (DoE)
2.5. The Identification of Optimized Electrospun Nanofibers
2.5.1. Scanning Electron Microscopy (SEM)
2.5.2. XRPD
2.5.3. Fourier Transform Infrared Spectroscopy with Attenuated Total Reflectance (FTIR-ATR)
2.6. Characterisation of Electrospun Nanofibers
2.6.1. Determination of Active Components Content
2.6.2. Dissolution Studies
2.6.3. Permeability Studies
2.6.4. In Vitro Assessment of Mucin–Biopolymer Bioadhesive Bond Strength
2.6.5. Antioxidant Activity
2.7. Statistical Analysis
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No. | % of Methanol in the Extraction Mixture | Temperature | Number of Cycles |
---|---|---|---|
W1 | 0 | 30 | 3 |
W2 | 0 | 50 | 5 |
W3 | 0 | 70 | 4 |
W4 | 35 | 30 | 5 |
W5 | 35 | 50 | 4 |
W6 | 35 | 70 | 3 |
W7 | 70 | 30 | 4 |
W8 | 70 | 50 | 3 |
W9 | 70 | 70 | 5 |
No. | Content | ||
---|---|---|---|
W10 Extract (g) | HPβCD (g) | PVP (g) | |
F1 | 1 | 1 | 1 |
F2 | 1 | 2 | 3 |
F3 | 1 | 3 | 2 |
F4 | 2 | 1 | 3 |
F5 | 2 | 2 | 2 |
F6 | 2 | 3 | 1 |
F7 | 3 | 1 | 2 |
F8 | 3 | 2 | 1 |
F9 | 3 | 3 | 3 |
No. | Content (µg/1 g Plant Material) Mean ± SD | Sum of Active Compounds (µg/1 g Plant Material) Mean | TPC (mg GAE/1 g Plant Material) Mean ± SD | |||
---|---|---|---|---|---|---|
Polydatin | Resveratrol | Emodin | Parietin | |||
W1 | 174.29 ± 0.68 | 185.85 ± 10.75 | 7.85 ± 0.41 | 0.08 ± 0.02 | 452.61 | 7.85 ± 0.41 |
W2 | 288.76 ± 22.64 | 206.69 ± 3.99 | 11.97 ± 0.62 | 0.23 ± 0.02 | 729.16 | 11.97 ± 0.62 |
W3 | 1384.68 ± 6.51 | 307.38 ± 1.57 | 12.95 ± 1.95 | 0.18 ± 0.01 | 1892.28 | 12.95 ± 1.95 |
W4 | 1060.43 ± 8.74 | 324.95 ± 15.49 | 20.35 ± 0.72 | 0.19 ± 0.08 | 1631.68 | 20.35 ± 0.72 |
W5 | 1388.60 ± 66.33 | 353.33 ± 12.53 | 21.96 ± 0.88 | 0.20 ± 0.01 | 2001.47 | 21.96 ± 0.88 |
W6 | 2891.96 ± 26.31 | 369.26 ± 3.88 | 23.11 ± 1.06 | 0.15 ± 0.02 | 3738.75 | 23.11 ± 1.06 |
W7 | 2730.72 ± 30.61 | 414.12 ± 5.30 | 23.09 ± 2.22 | 0.29 ± 0.02 | 3931.77 | 23.09 ± 2.22 |
W8 | 4179.04 ± 29.44 | 449.50 ± 1.24 | 30.75 ± 2.14 | 0.13 ± 0.08 | 4925.62 | 30.75 ± 2.14 |
W9 | 4199.43 ± 68.10 | 652.67 ± 17.44 | 35.01 ± 1.77 | 0.14 ± 0.02 | 5241.30 | 35.01 ± 1.77 |
No. | DPPH IC50 (mg/mL) | ABTS IC50 (mg/mL) | CUPRAC IC50 (mg/mL) | FRAP IC50 (mg/mL) | Hyaluronidase Inhibition IC50 (mg/mL) |
---|---|---|---|---|---|
Mean ± SD | |||||
W1 | 0.80 ± 0.02 | 0.66 ± 0.08 | 2.16 ± 0.02 | 0.49 ± 0.03 | 73.52 ± 2.37 |
W2 | 0.65 ± 0.01 | 0.49 ± 0.04 | 1.63 ± 0.03 | 0.30 ± 0.01 | 44.02 ± 1.48 |
W3 | 0.50 ± 0.01 | 0.42 ± 0.04 | 1.33 ± 0.30 | 0.29 ± 0.01 | 33.21 ± 2.40 |
W4 | 0.45 ± 0.01 | 0.44 ± 0.04 | 0.45 ± 0.02 | 0.23 ± 0.01 | 25.45 ± 0.87 |
W5 | 0.31 ± 0.02 | 0.38 ± 0.03 | 0.30 ± 0.02 | 0.18 ± 0.01 | 19.98 ± 1.67 |
W6 | 0.25 ± 0.01 | 0.31 ± 0.02 | 0.27 ± 0.03 | 0.13 ± 0.01 | 11.34 ± 2.45 |
W7 | 0.22 ± 0.03 | 0.21 ± 0.04 | 0.19 ± 0.01 | 0.14 ± 0.01 | 10.43 ± 1.33 |
W8 | 0.16 ± 0.02 | 0.18 ± 0.02 | 0.13 ± 0.01 | 0.11 ± 0.01 | 4.69 ± 0.33 |
W9 | 0.13 ± 0.01 | 0.14 ± 0.01 | 0.11 ± 0.01 | 0.09 ± 0.01 | 4.35 ± 0.28 |
IC50 (µg/mL) | IC50 (µg/mL) | IC50 (µg/mL) | IC50 (µg/mL) | ||
Resveratrol | 22.32 ± 0.20 | 10.84 ± 0.23 | 20.82 ± 1.29 | 9.17 ± 0.68 | |
Polydatin | 35.06 ± 1.11 | 25.21 ± 2.32 | 68.73 ± 0.48 | 12.90 ± 0.29 |
Sample | PVP | HPβCD | Lyophilized Extract | Nanofiber HPBCD-PVP | Nanofiber no. 5 |
---|---|---|---|---|---|
(1) Peak position [2θ] | 11.45 | 10.26 | - | 11.11 | 9.48 |
(2) Peak position [2θ] | 21.28 | 18.72 | 21.37 | 20.61 | 21.16 |
Matrix peak position displacement [2θ] | - | - | - | (1) −0.34 (2) −0.67 | (1) −1.97 (2) −0.12 |
Matrix peak position displacement [Å] | - | (1) 0.24 (2) 0.13 | (1) 1.76 (2) 0.02 |
Nanofibers 2 | Nanofibers 3 | Nanofibers 4 | Nanofibers 5 | Nanofibers 6 | Nanofibers 9 | |
---|---|---|---|---|---|---|
Content (µg/100 mg Nanofibers) | ||||||
Solvent: methanol | ||||||
Polydatin | 5.16 ± 0.11 | 8.35 ± 0.11 | 13.66 ± 0.87 | 33.32 ± 1.60 | 15.04 ± 3.48 | 9.45 ± 0.33 |
Resveratrol | 3.70 ± 0.01 | 1.28 ± 0.01 | 5.93 ± 0.59 | 8.60 ± 0.39 | 1.68 ± 0.33 | 1.05 ± 0.04 |
Solvent: artificial saliva solution at pH 6.8 | ||||||
Polydatin | 8.20 ± 0.01 | 6.96 ± 0.06 | 15.46 ± 0.01 | 27.46 ± 1.08 | 18.62 ± 0.27 | 19.24 ± 0.08 |
Resveratrol | 0.81 ± 0.03 | 0.60 ± 0.03 | 1.02 ± 0.01 | 11.00 ± 0.52 | 1.79 ± 0.02 | 2.26 ± 0.05 |
Nanofibers 2 | Nanofibers 3 | Nanofibers 4 | Nanofibers 5 | Nanofibers 6 | Nanofibers 9 | |
---|---|---|---|---|---|---|
Total Amount of Released Drug from 100 mg of Nanofibers (µg) at 15 min | ||||||
Polydatin | 5.75 ± 0.07 | 5.83 ± 0.04 | 12.50 ± 0.03 | 16.56 ± 0.22 | 18.80 ± 0.29 | 17.47 ± 0.02 |
Resveratrol | 0.31 ± 0.01 | 0 | 0.31 ± 0.02 | 3.80 ± 0.20 | 0.26 ± 0.01 | 0.56 ± 0.01 |
Standards | W10 | Nanofibers 2 | Nanofibers 3 | Nanofibers 4 | Nanofibers 5 | Nanofibers 6 | Nanofibers 9 | |
---|---|---|---|---|---|---|---|---|
Apparent Permeability Coefficient Papp × 10−6 (cm/s) | ||||||||
Polydatin | 0.0036 ± 0.0001 | 0.0096 ± 0.0007 | 3.6213 ± 0.4921 | 0.7235 ± 0.0460 | 0.1740 ± 0.0043 | 0.2060 ± 0.0155 | 0.0455 ± 0.0039 | 0.0088 ± 0.0003 |
Resveratrol | 1.0924 ± 0.0778 | 0.0281 ± 0.0017 | 47.5107 ± 6.1468 | 11.7056 ± 0.6146 | 11.3306 ± 0.2093 | 13.4603 ± 0.9008 | 0.1387 ± 0.0070 | 0.1102 ± 0.0042 |
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Paczkowska-Walendowska, M.; Miklaszewski, A.; Cielecka-Piontek, J. Is It Possible to Improve the Bioavailability of Resveratrol and Polydatin Derived from Polygoni cuspidati Radix as a Result of Preparing Electrospun Nanofibers Based on Polyvinylpyrrolidone/Cyclodextrin? Nutrients 2022, 14, 3897. https://doi.org/10.3390/nu14193897
Paczkowska-Walendowska M, Miklaszewski A, Cielecka-Piontek J. Is It Possible to Improve the Bioavailability of Resveratrol and Polydatin Derived from Polygoni cuspidati Radix as a Result of Preparing Electrospun Nanofibers Based on Polyvinylpyrrolidone/Cyclodextrin? Nutrients. 2022; 14(19):3897. https://doi.org/10.3390/nu14193897
Chicago/Turabian StylePaczkowska-Walendowska, Magdalena, Andrzej Miklaszewski, and Judyta Cielecka-Piontek. 2022. "Is It Possible to Improve the Bioavailability of Resveratrol and Polydatin Derived from Polygoni cuspidati Radix as a Result of Preparing Electrospun Nanofibers Based on Polyvinylpyrrolidone/Cyclodextrin?" Nutrients 14, no. 19: 3897. https://doi.org/10.3390/nu14193897