Mechanisms of Sodium/Iodide Symporter-Mediated Mammary Gland Iodine Compensation during Lactation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Treatments
2.2. Urine
2.3. Rats’ Milk
2.4. Measurement of Iodine in the Mammary Gland, Milk, Urine, and Serum
2.5. Thyroid Function Tests
2.6. HE Staining
2.7. Western Blot Analysis
2.8. Immunohistochemistry
2.9. Statistical Analysis
3. Results
3.1. Iodine Nutrition and Thyroid Hormone Levels in Lactating Rats
3.2. Effects of Different Iodine Intakes on Breast Milk Iodine Concentration and Mammary Gland Iodine Content in Lactating Rats
3.3. Effects of Iodine on Mammary Gland Cell Morphology in Lactating Rats
3.4. Effect of Iodine on NIS Expression in the Mammary Gland in Lactating Rats
3.5. Effect of Iodine on NIS Glycosylation and Degradation in the Mammary Gland during Lactation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | N | Serum | Urine | |
---|---|---|---|---|
STIC (μg/L) | SNBIC (μg/L) | UIC (μg/L) a | ||
LI | 6 | 29.12 (25.08, 33.27) | 19.79 (18.61, 21.66) | 87.45 (78.71, 95.64) |
NI | 6 | 55.77 (42.10, 71.76) | 54.33 (41.95, 63.48) | 369.96 (312.10, 433.28) |
10HI | 6 | 338.18 (218.43, 368.25) | 257.19 (220.31, 293.38) | 3569.18 (3287.96, 3793.56) |
50HI | 6 | 1090.08 (981.04, 1199.83) # | 841.83 (758.49, 903.45) # | 12,286.04 (11,470.80,13,362.59) # |
Group | N | BMIC (μg/L) a | MIC (μg) |
---|---|---|---|
LI | 6 | 26.46 (22.91, 44.91) | 0.38 (0.29, 0.50) |
NI | 6 | 220.46 (160.61, 224.36) | 0.81 (0.74, 1.37) |
10HI | 6 | 1188.75 (936.76, 1473.97) | 6.30 (2.37, 8.10) |
50HI | 6 | 7413.31 (4226.06, 9175.44) # | 72.19 (56.71, 99.63) # |
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Fu, M.; Gao, Y.; Guo, W.; Meng, Q.; Jin, Q.; Yang, R.; Yang, Y.; Zhang, Y.; Zhang, W. Mechanisms of Sodium/Iodide Symporter-Mediated Mammary Gland Iodine Compensation during Lactation. Nutrients 2022, 14, 3592. https://doi.org/10.3390/nu14173592
Fu M, Gao Y, Guo W, Meng Q, Jin Q, Yang R, Yang Y, Zhang Y, Zhang W. Mechanisms of Sodium/Iodide Symporter-Mediated Mammary Gland Iodine Compensation during Lactation. Nutrients. 2022; 14(17):3592. https://doi.org/10.3390/nu14173592
Chicago/Turabian StyleFu, Min, Yuanpeng Gao, Wenxing Guo, Qi Meng, Qi Jin, Rui Yang, Ying Yang, Yaqi Zhang, and Wanqi Zhang. 2022. "Mechanisms of Sodium/Iodide Symporter-Mediated Mammary Gland Iodine Compensation during Lactation" Nutrients 14, no. 17: 3592. https://doi.org/10.3390/nu14173592