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Nutrients
Volume 14
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10.3390/nu14153130
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Article

Alterations in Intestinal Brush Border Membrane Functionality and Bacterial Populations Following Intra-Amniotic Administration (Gallus gallus) of Nicotinamide Riboside and Its Derivatives

by
Nikolai Kolba
†,
Amin Zarei
†,
Jacquelyn Cheng
,
Nikita Agarwal
,
Younas Dadmohammadi
,
Leila Khazdooz
,
Alireza Abbaspourrad
* and
Elad Tako
*
Department of Food Science, Cornell University, Ithaca, NY 14853, USA
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Nutrients 2022, 14(15), 3130; https://doi.org/10.3390/nu14153130
Submission received: 26 April 2022 / Revised: 26 July 2022 / Accepted: 27 July 2022 / Published: 29 July 2022
(This article belongs to the Special Issue Emerging Dietary Bioactives in Health and Disease)
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Abstract

Nicotinamide riboside (NR) acts as a nicotinamide adenine dinucleotide (NAD+) precursor where NR supplementation has previously been shown to be beneficial. Thus, we synthesized and characterized nicotinamide riboside tributyrate chloride (NRTBCl, water-soluble) and nicotinamide riboside trioleate chloride (NRTOCl, oil-soluble) as two new ester derivatives of nicotinamide riboside chloride (NRCl). NRCl and its derivatives were assessed in vivo, via intra-amniotic administration (Gallus gallus), with the following treatment groups: (1) non-injected (control); and injection of (2) deionized H2O (control); (3) NRCl (30 mg/mL dose); (4) NRTBCl (30 mg/mL dose); and (5) NRTOCl (30 mg/mL dose). Post-intervention, the effects on physiological markers associated with brush border membrane morphology, intestinal bacterial populations, and duodenal gene expression of key proteins were investigated. Although no significant changes were observed in average body weights, NRTBCl exposure increased average cecum weight. NR treatment significantly increased Clostridium and NRCl treatment resulted in increased populations of Bifidobacterium, Lactobacillus, and E. coli. Duodenal gene expression analysis revealed that NRCl, NRTBCl, and NRTOCl treatments upregulated the expression of ZnT1, MUC2, and IL6 compared to the controls, suggesting alterations in brush border membrane functionality. The administration of NRCl and its derivatives appears to trigger increased expression of brush border membrane digestive proteins, with added effects on the composition and function of cecal microbial populations. Additional research is now warranted to further elucidate the effects on inflammatory biomarkers and observe changes in the specific intestinal bacterial populations post introduction of NR and its derivatives.
Keywords: intra-amniotic administration; brush border membrane; nicotinamide riboside derivatives; microbiome intra-amniotic administration; brush border membrane; nicotinamide riboside derivatives; microbiome

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MDPI and ACS Style

Kolba, N.; Zarei, A.; Cheng, J.; Agarwal, N.; Dadmohammadi, Y.; Khazdooz, L.; Abbaspourrad, A.; Tako, E. Alterations in Intestinal Brush Border Membrane Functionality and Bacterial Populations Following Intra-Amniotic Administration (Gallus gallus) of Nicotinamide Riboside and Its Derivatives. Nutrients 2022, 14, 3130. https://doi.org/10.3390/nu14153130

AMA Style

Kolba N, Zarei A, Cheng J, Agarwal N, Dadmohammadi Y, Khazdooz L, Abbaspourrad A, Tako E. Alterations in Intestinal Brush Border Membrane Functionality and Bacterial Populations Following Intra-Amniotic Administration (Gallus gallus) of Nicotinamide Riboside and Its Derivatives. Nutrients. 2022; 14(15):3130. https://doi.org/10.3390/nu14153130

Chicago/Turabian Style

Kolba, Nikolai, Amin Zarei, Jacquelyn Cheng, Nikita Agarwal, Younas Dadmohammadi, Leila Khazdooz, Alireza Abbaspourrad, and Elad Tako. 2022. "Alterations in Intestinal Brush Border Membrane Functionality and Bacterial Populations Following Intra-Amniotic Administration (Gallus gallus) of Nicotinamide Riboside and Its Derivatives" Nutrients 14, no. 15: 3130. https://doi.org/10.3390/nu14153130

APA Style

Kolba, N., Zarei, A., Cheng, J., Agarwal, N., Dadmohammadi, Y., Khazdooz, L., Abbaspourrad, A., & Tako, E. (2022). Alterations in Intestinal Brush Border Membrane Functionality and Bacterial Populations Following Intra-Amniotic Administration (Gallus gallus) of Nicotinamide Riboside and Its Derivatives. Nutrients, 14(15), 3130. https://doi.org/10.3390/nu14153130

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