Next Article in Journal
Effects of Probiotic NVP-1704 on Mental Health and Sleep in Healthy Adults: An 8-Week Randomized, Double-Blind, Placebo-Controlled Trial
Next Article in Special Issue
Lupin-Derived Bioactive Peptides: Intestinal Transport, Bioavailability and Health Benefits
Previous Article in Journal
Associations between Infant Dietary Intakes and Liking for Sweetness and Fattiness Sensations in 8-to-12-Year-Old Children
Previous Article in Special Issue
Anti-Obesity and Hypocholesterolemic Actions of Protamine-Derived Peptide RPR (Arg-Pro-Arg) and Protamine in High-Fat Diet-Induced C57BL/6J Mice
Article

Anti-Salmonella Activity and Peptidomic Profiling of Peptide Fractions Produced from Sturgeon Fish Skin Collagen (Huso huso) Using Commercial Enzymes

1
Department of Seafood Science and Technology, Faculty of Fisheries and Environmental Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 4913815739, Iran
2
School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1H 8M5, Canada
3
Applied Biotechnology Research Center, Tehran 19945-546, Iran
4
Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari 48181-68984, Iran
5
Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada
*
Authors to whom correspondence should be addressed.
Academic Editor: Carmen Lammi
Nutrients 2021, 13(8), 2657; https://doi.org/10.3390/nu13082657
Received: 1 July 2021 / Revised: 23 July 2021 / Accepted: 26 July 2021 / Published: 30 July 2021
(This article belongs to the Special Issue Bioactive Peptides)
This study investigated peptide fractions from fish skin collagen for antibacterial activity against Escherichia coli and Salmonella strains. The collagen was hydrolyzed with six commercial proteases, including trypsin, Alcalase, Neutrase, Flavourzyme, pepsin and papain. Hydrolyzed samples obtained with trypsin and Alcalase had the largest number of small peptides (molecular weight <10 kDa), while the hydrolysate produced with papain showed the lowest degree of hydrolysis and highest number of large peptides. Four hydrolysates were found to inhibit the growth of the Gram-negative bacteria, with papain hydrolysate showing the best activity against E. coli, and Neutrase and papain hydrolysates showing the best activity against S. abony; hydrolysates produced with trypsin and pepsin did not show detectable antibacterial activity. After acetone fractionation of the latter hydrolysates, the peptide fractions demonstrated enhanced dose-dependent inhibition of the growth (colony-forming units) of four Salmonella strains, including S. abony (NCTC 6017), S. typhimurium (ATCC 13311), S. typhimurium (ATCC 14028) and S. chol (ATCC 10708). Shotgun peptidomics analysis of the acetone fractions of Neutrase and papain hydrolysates resulted in the identification of 71 and 103 peptides, respectively, with chain lengths of 6–22 and 6–24, respectively. This work provided an array of peptide sequences from fish skin collagen for pharmacophore identification, structure–activity relationship studies, and further investigation as food-based antibacterial agents against pathogenic microorganisms. View Full-Text
Keywords: sturgeon fish (Huso huso); collagen; bioactive peptides; antimicrobial peptides; peptidomics; Salmonella sturgeon fish (Huso huso); collagen; bioactive peptides; antimicrobial peptides; peptidomics; Salmonella
Show Figures

Figure 1

MDPI and ACS Style

Atef, M.; Chait, Y.A.; Ojagh, S.M.; Latifi, A.M.; Esmaeili, M.; Hammami, R.; Udenigwe, C.C. Anti-Salmonella Activity and Peptidomic Profiling of Peptide Fractions Produced from Sturgeon Fish Skin Collagen (Huso huso) Using Commercial Enzymes. Nutrients 2021, 13, 2657. https://doi.org/10.3390/nu13082657

AMA Style

Atef M, Chait YA, Ojagh SM, Latifi AM, Esmaeili M, Hammami R, Udenigwe CC. Anti-Salmonella Activity and Peptidomic Profiling of Peptide Fractions Produced from Sturgeon Fish Skin Collagen (Huso huso) Using Commercial Enzymes. Nutrients. 2021; 13(8):2657. https://doi.org/10.3390/nu13082657

Chicago/Turabian Style

Atef, Maryam, Yasmina A. Chait, Seyed M. Ojagh, Ali M. Latifi, Mina Esmaeili, Riadh Hammami, and Chibuike C. Udenigwe 2021. "Anti-Salmonella Activity and Peptidomic Profiling of Peptide Fractions Produced from Sturgeon Fish Skin Collagen (Huso huso) Using Commercial Enzymes" Nutrients 13, no. 8: 2657. https://doi.org/10.3390/nu13082657

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop