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Purified Gymnemic Acids from Gymnema inodorum Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes

Cellular and Molecular Biotechnology Research Institute, National Institute of Advance Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan
Department of Environment and Natural Resources, Okinawa Industrial Technology Center, Okinawa 904-2234, Japan
Research and Development Group for Bio-Industries, Thailand Institute of Scientific and Technological Research (TISTR), Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand
Author to whom correspondence should be addressed.
Nutrients 2020, 12(9), 2851;
Received: 20 August 2020 / Revised: 14 September 2020 / Accepted: 15 September 2020 / Published: 17 September 2020
(This article belongs to the Special Issue Nutraceuticals and Human Health)
Gymnema inodorum (GI) is an indigenous medicinal plant and functional food in Thailand that has recently helped to reduce plasma glucose levels in healthy humans. It is renowned for the medicinal properties of gymnemic acid and its ability to suppress glucose absorption. However, the effects of gymnemic acids on adipogenesis that contribute to the accumulation of adipose tissues associated with obesity remain unknown. The present study aimed to determine the effects of gymnemic acids derived from GI tea on adipogenesis. We purified and identified GiA-7 and stephanosides C and B from GI tea that inhibited adipocyte differentiation in 3T3-L1 cells. These compounds also suppressed the expression of peroxisome proliferator-activated receptor gamma (Pparγ)-dependent genes, indicating that they inhibit lipid accumulation and the early stage of 3T3-L1 preadipocyte differentiation. Only GiA-7 induced the expression of uncoupling protein 1 (Ucp1) and pparγ coactivator 1 alpha (Pgc1α), suggesting that GiA-7 induces mitochondrial activity and beige-like adipocytes. This is the first finding of stephanosides C and B in Gymnema inodorum. Our results suggested that GiA-7 and stephanosides C and B from GI tea could help to prevent obesity. View Full-Text
Keywords: Gymnema inodorum; adipogenesis; gymnemic acid; obesity Gymnema inodorum; adipogenesis; gymnemic acid; obesity
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Saiki, P.; Kawano, Y.; Ogi, T.; Klungsupya, P.; Muangman, T.; Phantanaprates, W.; Kongchinda, P.; Pinnak, N.; Miyazaki, K. Purified Gymnemic Acids from Gymnema inodorum Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes. Nutrients 2020, 12, 2851.

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