Next Article in Journal
Effects of Dietary Nitrates on Time Trial Performance in Athletes with Different Training Status: Systematic Review
Next Article in Special Issue
The “Fortilat” Randomized Clinical Trial Follow-Up: Auxological Outcome at 18 Months of Age
Previous Article in Journal
Correction: Yao, C.A., et al. Soy-Based Multiple Amino Acid Oral Supplementation Increases the Anti-Sarcoma Effect of Cyclophosphamide. Nutrients 2016, 8, 192
Previous Article in Special Issue
Urinary Metabolomic Profile of Preterm Infants Receiving Human Milk with Either Bovine or Donkey Milk-Based Fortifiers
Open AccessArticle

Impact of Storage Conditions on the Breast Milk Peptidome

1
Department of Neonatology and Paediatric Intensive Care, University Medicine Greifswald, D-17475 Greifswald, Germany
2
Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, D-17475 Greifswald, Germany
3
Department of Neurology, University Medicine Greifswald, D-17475 Greifswald, Germany
4
Department of Psychiatry & Psychotherapy, University Medicine Greifswald, D-17475 Greifswald, Germany
*
Authors to whom correspondence should be addressed.
Nutrients 2020, 12(9), 2733; https://doi.org/10.3390/nu12092733
Received: 28 July 2020 / Revised: 28 August 2020 / Accepted: 4 September 2020 / Published: 8 September 2020
(This article belongs to the Special Issue Donkey Milk and Infant Nutrition)
Human donor milk (HDM) provides appropriate nutrition and offers protective functions in preterm infants. The aim of the study is to examine the impact of different storage conditions on the stability of the human breast milk peptidome. HDM was directly frozen at −80 °C or stored at −20 °C (120 h), 4 °C (6 h), or room temperature (RT for 6 or 24 h). The milk peptidome was profiled by mass spectrometry after peptide collection by ultrafiltration. Profiling of the peptidome covered 3587 peptides corresponding to 212 proteins. The variance of the peptidome increased with storage temperature and time and varied for different peptides. The highest impact was observed when samples were stored at RT. Smaller but significant effects were still observed in samples stored at 4 °C, while samples showed highest similarity to those immediately frozen at −80 °C when stored at −20 °C. Peptide structures after storage at RT for 24 h point to the increased activity of thrombin and other proteases cleaving proteins at lysine/arginine. The results point to an ongoing protein degradation/peptide production by milk-derived proteases. They underline the need for immediate freezing of HDM at −20 °C or −80 °C to prevent degradation of peptides and enable reproducible investigation of prospectively collected samples. View Full-Text
Keywords: human breast milk; peptidome; LC-MS/MS; storage conditions; temperature human breast milk; peptidome; LC-MS/MS; storage conditions; temperature
Show Figures

Graphical abstract

MDPI and ACS Style

Howland, V.; Klaedtke, M.; Ruhnau, J.; Dhople, V.M.; Grabe, H.J.; Völker, U.; Heckmann, M.; Hammer, E. Impact of Storage Conditions on the Breast Milk Peptidome. Nutrients 2020, 12, 2733. https://doi.org/10.3390/nu12092733

AMA Style

Howland V, Klaedtke M, Ruhnau J, Dhople VM, Grabe HJ, Völker U, Heckmann M, Hammer E. Impact of Storage Conditions on the Breast Milk Peptidome. Nutrients. 2020; 12(9):2733. https://doi.org/10.3390/nu12092733

Chicago/Turabian Style

Howland, Vanessa; Klaedtke, Maik; Ruhnau, Johanna; Dhople, Vishnu M.; Grabe, Hans J.; Völker, Uwe; Heckmann, Matthias; Hammer, Elke. 2020. "Impact of Storage Conditions on the Breast Milk Peptidome" Nutrients 12, no. 9: 2733. https://doi.org/10.3390/nu12092733

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop