Gastrointestinally Digested Protein from the Insect Alphitobius diaperinus Stimulates a Different Intestinal Secretome than Beef or Almond, Producing a Differential Response in Food Intake in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. In Vitro Digestion
2.2.1. Gastric Digestion
2.2.2. Intestinal Digestion
2.3. Characterization of Digestion Products
2.4. Tissue Collection
2.4.1. Pig Tissue
2.4.2. Human Tissue
2.5. Ex Vivo Experiments with Intestinal Segments
2.6. Food Intake Study in Rats
2.7. Statistical Analysis
3. Results
3.1. Characterization of Digested Samples
3.2. Insect Gastric Digestion Stimulated Pig Duodenal Enteroendocrine Secretions Differently than Beef or Almond
3.3. Insect Intestinal Digestion Induced GLP1 Secretion and Reduced Ghrelin Secretion in Human Colon Samples
3.4. Buffalo Alphitobius diaperinus Powder Stimulated Food Intake in Rats
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Samples | Protein mg/mL | Triglycerides mg/mL | Glucose mg/mL | |
---|---|---|---|---|
Gastric digestion | Insect | 26.60 | 5.84 | 0.20 |
IPC | 31.49 | 15.30 | 0.22 | |
Beef | 21.60 | 0.92 | 0.09 | |
Almond | 26.55 | 2.24 | 0.12 | |
Enzymes | 0.65 | 0.05 | n.d. | |
Intestinal digestion | Insect | 15.02 | 8.97 | 0.09 |
IPC | 14.38 | 12.91 | 0.08 | |
Beef | 9.28 | 0.70 | 0.06 | |
Almond | 10.29 | 9.44 | 0.1 | |
Enzymes | 0.62 | 0.05 | n.d. |
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Miguéns-Gómez, A.; Grau-Bové, C.; Sierra-Cruz, M.; Jorba-Martín, R.; Caro, A.; Rodríguez-Gallego, E.; Beltrán-Debón, R.; Blay, M.T.; Terra, X.; Ardévol, A.; et al. Gastrointestinally Digested Protein from the Insect Alphitobius diaperinus Stimulates a Different Intestinal Secretome than Beef or Almond, Producing a Differential Response in Food Intake in Rats. Nutrients 2020, 12, 2366. https://doi.org/10.3390/nu12082366
Miguéns-Gómez A, Grau-Bové C, Sierra-Cruz M, Jorba-Martín R, Caro A, Rodríguez-Gallego E, Beltrán-Debón R, Blay MT, Terra X, Ardévol A, et al. Gastrointestinally Digested Protein from the Insect Alphitobius diaperinus Stimulates a Different Intestinal Secretome than Beef or Almond, Producing a Differential Response in Food Intake in Rats. Nutrients. 2020; 12(8):2366. https://doi.org/10.3390/nu12082366
Chicago/Turabian StyleMiguéns-Gómez, Alba, Carme Grau-Bové, Marta Sierra-Cruz, Rosa Jorba-Martín, Aleidis Caro, Esther Rodríguez-Gallego, Raúl Beltrán-Debón, M Teresa Blay, Ximena Terra, Anna Ardévol, and et al. 2020. "Gastrointestinally Digested Protein from the Insect Alphitobius diaperinus Stimulates a Different Intestinal Secretome than Beef or Almond, Producing a Differential Response in Food Intake in Rats" Nutrients 12, no. 8: 2366. https://doi.org/10.3390/nu12082366