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Article

Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice

by 1,2, 1,2, 1,2, 1,2, 1,2, 1,2 and 1,2,*
1
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
2
Beijing Key Laboratory of Biological Feed Additive, China Agricultural University, Beijing 100193, China
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(8), 2298; https://doi.org/10.3390/nu12082298
Received: 9 July 2020 / Revised: 24 July 2020 / Accepted: 27 July 2020 / Published: 31 July 2020
Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota. We examined whether L. reuteri I5007 could protect mice against colitis in ameliorating inflammation, modulating microbiota, and metabolic composition. In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment. Pretreatment with L. reuteri I5007 effectively relieves inflammation in HT-29 cells challenged with lipopolysaccharide. In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS. L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels. Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition. Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis. View Full-Text
Keywords: DSS-induced colitis; gut microbiota; IBD; Lactobacillus reuteri; metabolites DSS-induced colitis; gut microbiota; IBD; Lactobacillus reuteri; metabolites
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MDPI and ACS Style

Wang, G.; Huang, S.; Cai, S.; Yu, H.; Wang, Y.; Zeng, X.; Qiao, S. Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice. Nutrients 2020, 12, 2298. https://doi.org/10.3390/nu12082298

AMA Style

Wang G, Huang S, Cai S, Yu H, Wang Y, Zeng X, Qiao S. Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice. Nutrients. 2020; 12(8):2298. https://doi.org/10.3390/nu12082298

Chicago/Turabian Style

Wang, Gang, Shuo Huang, Shuang Cai, Haitao Yu, Yuming Wang, Xiangfang Zeng, and Shiyan Qiao. 2020. "Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice" Nutrients 12, no. 8: 2298. https://doi.org/10.3390/nu12082298

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