Anti-Adipogenic Effect of Neferine in 3T3-L1 Cells and Primary White Adipocytes
1
Department of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Korea
2
Institute for Aging and Clinical Nutrition Research, Gachon University, Gyeonggi-do 13120, Korea
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(6), 1858; https://doi.org/10.3390/nu12061858
Received: 25 May 2020
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Revised: 11 June 2020
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Accepted: 17 June 2020
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Published: 22 June 2020
(This article belongs to the Section Phytochemicals and Human Health)
Neferine, an alkaloid component extracted from lotus seed embryos, is known for its anti-inflammatory, anticancer, and antioxidant properties. However, the anti-adipogenic activity of neferine has not been thoroughly investigated. In this study, neferine was found to inhibit lipid accumulation in a dose-dependent manner during the differentiation of 3T3-L1 cells without inducing cytotoxicity. Real-time polymerase chain reaction and immunoblot analysis revealed the downregulation in the expression of peroxisome proliferator activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), sterol regulatory element-binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) and the upregulation in carnitine palmitoyltransferase-1 (CPT-1) and sirtuin 1 (SIRT1) levels following neferine treatment. Furthermore, neferine increased the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), which is an important regulator of fatty acid oxidation. Our result indicates that neferine attenuates adipogenesis and promotes lipid metabolism by activating AMPK-mediated signaling. Therefore, neferine may serve as a therapeutic candidate for obesity treatment.
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MDPI and ACS Style
Park, M.; Han, J.; Lee, H.-J. Anti-Adipogenic Effect of Neferine in 3T3-L1 Cells and Primary White Adipocytes. Nutrients 2020, 12, 1858. https://doi.org/10.3390/nu12061858
AMA Style
Park M, Han J, Lee H-J. Anti-Adipogenic Effect of Neferine in 3T3-L1 Cells and Primary White Adipocytes. Nutrients. 2020; 12(6):1858. https://doi.org/10.3390/nu12061858
Chicago/Turabian StylePark, Miey, Jinyoung Han, and Hae-Jeung Lee. 2020. "Anti-Adipogenic Effect of Neferine in 3T3-L1 Cells and Primary White Adipocytes" Nutrients 12, no. 6: 1858. https://doi.org/10.3390/nu12061858
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