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The In Vitro Analysis of Prebiotics to Be Used as a Component of a Synbiotic Preparation

Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland
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Nutrients 2020, 12(5), 1272; https://doi.org/10.3390/nu12051272
Received: 6 March 2020 / Revised: 27 April 2020 / Accepted: 29 April 2020 / Published: 30 April 2020
(This article belongs to the Section Prebiotics and Probiotics)
Prebiotics are food components that are selectively fermented by beneficial microbiota and which confer a health benefit. The aim of the study was to select a prebiotic for the chosen probiotic strains to create a synbiotic. The impact of prebiotics (inulin, maltodextrin, corn starch, β-glucan, and apple pectin) on five Lactobacillus spp. strains’ growth and metabolites synthesis (lactic, acetic, propionic, and butyric acids, ethanol, and acetaldehyde) was tested by the plate count method and by high-performance liquid chromatography, respectively. Moreover, the differences in the ratio of D(−) and L(+) lactate isomers produced by Lactobacillus spp., as well as variations in the probiotics’ enzymatic profiles associated with the prebiotic used for cultivation, were determined with a Megazyme rapid assay kit and API® ZYM assay, accordingly. Finally, the influence of the carbon source (prebiotic) used on the antagonistic activity of the probiotic strains towards pathogenic bacteria, such as Salmonella spp. or Listeria monocytogenes was analyzed in the co-cultures. The results showed that the growth, metabolic profile, and antagonistic activity of the probiotics towards selected pathogens were the most favorable when 2% (w/v) of inulin was used. Therefore, the combination of inulin with selected probiotics is a promising synbiotic mixture. View Full-Text
Keywords: probiotics; prebiotics; inulin; synbiotics; microbial growth; metabolic activity; antagonism probiotics; prebiotics; inulin; synbiotics; microbial growth; metabolic activity; antagonism
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Śliżewska, K.; Chlebicz-Wójcik, A. The In Vitro Analysis of Prebiotics to Be Used as a Component of a Synbiotic Preparation. Nutrients 2020, 12, 1272.

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