Potato Protein Isolate Stimulates Muscle Protein Synthesis at Rest and with Resistance Exercise in Young Women
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Participants
2.3. Study Overview
2.4. Baseline Testing
2.5. Diets
2.6. Resistance Exercise
2.7. Supplementation
2.8. Integrated Rates of Muscle Protein Synthesis
2.9. Analytical Methods
2.10. Western Blotting
2.11. Saliva Sample Analusis
2.12. Calculations
2.13. Statistics
3. Results
3.1. Participants’ Characteristics
3.2. Dietary Intake
3.3. Integrated Myofibrillar Protein Synthesis (MPS)
3.4. Muscle Signaling Protein Content
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Amino acid (g) | PP | CON |
---|---|---|
Isoleucine | 1.4 | - |
Leucine | 2.5 | - |
Valine | 1.6 | - |
Lysine | 1.8 | - |
Methionine | 0.6 | - |
Phenylalanine | 1.6 | - |
Threonine | 1.4 | - |
Tryptophan | 0.3 | - |
Histidine | 0.6 | - |
Cysteine | 0.4 | - |
Tyrosine | 1.3 | - |
Arginine | 1.3 | - |
Alanine | 1.2 | - |
Aspartic Acid + Asparagine | 2.9 | - |
Glutamic acid + Glutamine | 2.8 | - |
Glycine | 1.2 | - |
Proline | 1.2 | - |
Serine | 1.3 | - |
ΣEAA | 11.6 | - |
ΣNEAA | 13.5 | - |
Total energy per supplement (kcal) | 300 | 200 |
Carbohydrate (g) | 19 | 19 |
PP (n = 12) | CON (n = 12) | p | |
---|---|---|---|
Age (y) | 20 ± 3 | 21 ± 3 | 0.41 |
Height (m) | 1.68 ± 0.44 | 1.64 ± 0.79 | 0.10 |
Body mass (kg) | 64.4 ± 8.2 | 61.9 ± 11.4 | 0.55 |
BMI (kg/m2) | 22.8 ± 2.3 | 23.1 ± 3.0 | 0.78 |
Body fat, (%) | 28.4 ± 5.9 | 31.5 ± 5.5 | 0.20 |
LBM (kg) | 42.9 ± 3.5 | 39.8 ± 6.6 | 0.16 |
Knee extensor 1RM (kg) | 44 ± 8 | 36 ± 7 | 0.08 |
Knee extensor 1RM, per kg body mass (kg) | 0.69 ± 0.13 | 0.58 ± 0.07 | 0.08 |
Leg press 1RM (kg) | 86 ± 29 | 68 ± 18 | 0.07 |
Leg press 1RM, per kg body mass (kg) | 1.34 ± 0.42 | 1.12 ± 0.33 | 0.02 |
PP (n = 12) | CON (n = 12) | p | |
---|---|---|---|
Protein (g/kg/d) | |||
Baseline | 0.80 ± 0.01 | 0.80 ± 0.01 | 0.68 |
Supplementation | 1.59 ± 0.09 * | 0.80 ± 0.01 | <0.001 |
Non-supplemental protein (% of total) | 51 ± 3 | 100 ± 0 | |
Energy (kcal/kg) | |||
Supplementation | 34 ± 3 | 33 ± 5 | 0.34 |
Carbohydrates (g/kg) | |||
Supplementation | 4.4 ± 0.5 | 5.0 ± 0.8 | 0.08 |
Fat (g/kg) | |||
Supplementation | 1.2 ± 0.1 | 1.0 ± 0.3 | 0.17 |
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Oikawa, S.Y.; Bahniwal, R.; Holloway, T.M.; Lim, C.; McLeod, J.C.; McGlory, C.; Baker, S.K.; Phillips, S.M. Potato Protein Isolate Stimulates Muscle Protein Synthesis at Rest and with Resistance Exercise in Young Women. Nutrients 2020, 12, 1235. https://doi.org/10.3390/nu12051235
Oikawa SY, Bahniwal R, Holloway TM, Lim C, McLeod JC, McGlory C, Baker SK, Phillips SM. Potato Protein Isolate Stimulates Muscle Protein Synthesis at Rest and with Resistance Exercise in Young Women. Nutrients. 2020; 12(5):1235. https://doi.org/10.3390/nu12051235
Chicago/Turabian StyleOikawa, Sara Y., Ravninder Bahniwal, Tanya M. Holloway, Changhyun Lim, Jonathan C. McLeod, Chris McGlory, Steven K. Baker, and Stuart M. Phillips. 2020. "Potato Protein Isolate Stimulates Muscle Protein Synthesis at Rest and with Resistance Exercise in Young Women" Nutrients 12, no. 5: 1235. https://doi.org/10.3390/nu12051235