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Open AccessArticle

Effects of Rambutan Peel (Nepheliumlappaceum) PhenolicExtract on RANKL-Induced Differentiation of RAW264.7 Cells into Osteoclasts and Retinoic Acid-Induced Osteoporosis in Rats

1
Institute of Agriculture and Food, Kunming University of Science and Technology, No. 727 South Jingming Road, Kunming 650500, China
2
Food Science and Technology, Ocean University of China, No 5, Yushan Road, Qingdao, Shandong 266005, China
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(4), 883; https://doi.org/10.3390/nu12040883 (registering DOI)
Received: 1 March 2020 / Revised: 19 March 2020 / Accepted: 20 March 2020 / Published: 25 March 2020
(This article belongs to the Section Phytochemicals and Human Health)
Previous studies have shown that rambutan peel phenolic (RPP) extract has excellent biological activities due to its abundant phenolic content and profile. In this study, the potential anti-osteoporosis (OP) effects of RPP were evaluated by suppressing receptor activator nuclear factor-kappa B ligand (RANKL)-induced differentiation of RAW264.7 cells into osteoclasts and amelioratingretinoic acid-induced OP in rats. Our results showed that RPP efficiently decreased the formation of tartrate-resistant acid phosphatase (TRAP)-positive cells and reduced total TRAP activity in RAW264.7 cells under RANKL stimulation. RPP treatment significantlyameliorated retinoid acid-induced calcium loss in rats (p < 0.05). The serum phosphorus level of osteoporotic rats was increased by RPP treatment, and the serum levels of total alkaline phosphatase and osteocalcin in osteoporotic rats were further reduced. RPP treatment improved the qualities of the femur and tibia, such asbone mineral density, bone length, bone maximum load, cortical bone area ratio, and trabecularelative bone density in osteoporotic rats to some extent. Furthermore, histological analysis showed that RPP effectively improved the bone microstructure of osteoporotic rats by regulating the cortical bone thickness and trabecular bone separation. These results indicate that RPP could have potential applications as a newnutraceutical and functional food in the prevention of OP. View Full-Text
Keywords: rambutanpeel phenolic; osteoporosis; RAW264.7 cells; receptor activator nuclear factor-kappa B ligand(RANKL); histological analysis; bone quality rambutanpeel phenolic; osteoporosis; RAW264.7 cells; receptor activator nuclear factor-kappa B ligand(RANKL); histological analysis; bone quality
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Zhuang, Y.; Sun, X.; Liu, B.; Hou, H.; Sun, Y. Effects of Rambutan Peel (Nepheliumlappaceum) PhenolicExtract on RANKL-Induced Differentiation of RAW264.7 Cells into Osteoclasts and Retinoic Acid-Induced Osteoporosis in Rats. Nutrients 2020, 12, 883.

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