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Open AccessArticle

Impact of Epicatechin on the Procoagulant Activities of Microparticles

1
UNH Unité de Nutrition Humaine, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Université Clermont Auvergne, 63000 Clermont-Ferrand, France
2
CHU Clermont-Ferrand, Service d’Hématologie Biologique, 63000 Clermont-Ferrand, France
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(10), 2935; https://doi.org/10.3390/nu12102935
Received: 6 August 2020 / Revised: 23 September 2020 / Accepted: 23 September 2020 / Published: 25 September 2020
(This article belongs to the Special Issue Dietary Polyphenols and Cardiometabolic Health)
Microparticles play a role in cardiovascular disease pathology. The flavanol-like epicatechin is increasingly considered due to its cardioprotective effects. The aim of this study was to investigate the impact of epicatechin on microparticle generation, phenotype and procoagulant properties. Plasma samples from 15 healthy subjects were incubated with increasing concentrations of epicatechin (1 to 100 μM). Then, the expression of glycoprotein IIb, phosphatidylserine (PS), glycoprotein Ib (GPIb) and P-selectin was assessed by flow cytometry analysis after (or not) platelet stimulation. Microparticle procoagulant activity was determined using ZymuphenTM MP and ZymuphenTM MP-TF for phospholipid and tissue factor content, and with thrombin generation (TG) assays for procoagulant function. Platelet microparticles that express GPIb (/µL) decreased from 20,743 ± 24,985 (vehicle) to 14,939 ± 14,333 (p = 0.6), 21,366 ± 16,949 (p = 0.9) and 15,425 ± 9953 (p < 0.05) in samples incubated with 1, 10 and 100 µM epicatechin, respectively. Microparticle concentration (nM PS) decreased from 5.6 ± 2.0 (vehicle) to 5.1 ± 2.2 (p = 0.5), 4.5 ± 1.5 (p < 0.05) and 4.7 ± 2.0 (p < 0.05) in samples incubated with 1, 10 and 100µM epicatechin, respectively. Epicatechin had no impact on tissue factor-positive microparticle concentration. Epicatechin decreased TG (endogenous thrombin potential, nM.min) from 586 ± 302 to 509 ± 226 (p = 0.3), 512 ± 270 (p = 0.3) and 445 ± 283 (p < 0.05). These findings indicate that epicatechin affects microparticle release, phenotype and procoagulant properties. View Full-Text
Keywords: cardiovascular disease; coagulation; epicatechin; hemostasis; microparticles cardiovascular disease; coagulation; epicatechin; hemostasis; microparticles
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MDPI and ACS Style

Sinegre, T.; Milenkovic, D.; Bourgne, C.; Teissandier, D.; Nasri, Y.; Dannus, L.-T.; Morand, C.; Lebreton, A. Impact of Epicatechin on the Procoagulant Activities of Microparticles. Nutrients 2020, 12, 2935. https://doi.org/10.3390/nu12102935

AMA Style

Sinegre T, Milenkovic D, Bourgne C, Teissandier D, Nasri Y, Dannus L-T, Morand C, Lebreton A. Impact of Epicatechin on the Procoagulant Activities of Microparticles. Nutrients. 2020; 12(10):2935. https://doi.org/10.3390/nu12102935

Chicago/Turabian Style

Sinegre, Thomas; Milenkovic, Dragan; Bourgne, Céline; Teissandier, Dorian; Nasri, Yosra; Dannus, Louis-Thomas; Morand, Christine; Lebreton, Aurélien. 2020. "Impact of Epicatechin on the Procoagulant Activities of Microparticles" Nutrients 12, no. 10: 2935. https://doi.org/10.3390/nu12102935

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