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Article

Lipophilized Epigallocatechin Gallate Derivative Exerts Anti-Proliferation Efficacy through Induction of Cell Cycle Arrest and Apoptosis on DU145 Human Prostate Cancer Cells

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
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Nutrients 2020, 12(1), 92; https://doi.org/10.3390/nu12010092
Received: 22 November 2019 / Revised: 16 December 2019 / Accepted: 25 December 2019 / Published: 28 December 2019
(This article belongs to the Section Phytochemicals and Human Health)
Epigallocatechin gallate (EGCG) is the predominant tea polyphenol and it exhibits a hydrophilic character. The lipophilized EGCG derivative (LEGCG) was synthesized by enzymatic esterification of EGCG with lauric acid to enhance its bioactivity. The tetralauroyl EGCG was confirmed by high-performance liquid chromatography-tandem mass spectrometry and further identified as 3′, 5′, 3″, 5″-4-O-lauroyl EGCG by 1H and 13C nuclear magnetic resonance. The anti-proliferation effect of LEGCG on DU145 human prostate carcinoma cells was evaluated by MTT assay. In addition, the underlying molecular mechanism by which LEGCG exerts anti-proliferation efficacy was elucidated by flow cytometry and immunoblot analysis. Results suggested that LEGCG exhibited a dose-dependent anti-proliferation effect on DU145 cells by G0/G1 phase arrest and induction of apoptosis. LEGCG induced cell cycle arrest via p53/p21 activation, which down-regulated the cyclin D1 and CDK4 expression. In addition, LEGCG induced apoptosis by increasing the Bax/Bcl-2 ratio, the cytochrome c release, and the caspases cleavage on DU145 cells. The results provide theoretical support to prevent prostate cancer with LEGCG. View Full-Text
Keywords: EGCG; LEGCG; DU145 cell; proliferation; apoptosis EGCG; LEGCG; DU145 cell; proliferation; apoptosis
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MDPI and ACS Style

Chen, J.; Zhang, L.; Li, C.; Chen, R.; Liu, C.; Chen, M. Lipophilized Epigallocatechin Gallate Derivative Exerts Anti-Proliferation Efficacy through Induction of Cell Cycle Arrest and Apoptosis on DU145 Human Prostate Cancer Cells. Nutrients 2020, 12, 92. https://doi.org/10.3390/nu12010092

AMA Style

Chen J, Zhang L, Li C, Chen R, Liu C, Chen M. Lipophilized Epigallocatechin Gallate Derivative Exerts Anti-Proliferation Efficacy through Induction of Cell Cycle Arrest and Apoptosis on DU145 Human Prostate Cancer Cells. Nutrients. 2020; 12(1):92. https://doi.org/10.3390/nu12010092

Chicago/Turabian Style

Chen, Jun, Linli Zhang, Changhong Li, Ruochen Chen, Chengmei Liu, and Mingshun Chen. 2020. "Lipophilized Epigallocatechin Gallate Derivative Exerts Anti-Proliferation Efficacy through Induction of Cell Cycle Arrest and Apoptosis on DU145 Human Prostate Cancer Cells" Nutrients 12, no. 1: 92. https://doi.org/10.3390/nu12010092

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