Next Article in Journal
Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible
Next Article in Special Issue
Stress-Reducing Function of Matcha Green Tea in Animal Experiments and Clinical Trials
Previous Article in Journal
Association between Cognitive Restraint, Uncontrolled Eating, Emotional Eating and BMI and the Amount of Food Wasted in Early Adolescent Girls
Previous Article in Special Issue
Epigallocatechin-3-gallate and 6-OH-11-O-Hydroxyphenanthrene Limit BE(2)-C Neuroblastoma Cell Growth and Neurosphere Formation In Vitro
Open AccessArticle

Polyphenols in Liubao Tea Can Prevent CCl4-Induced Hepatic Damage in Mice through Its Antioxidant Capacities

Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China
Author to whom correspondence should be addressed.
Nutrients 2018, 10(9), 1280;
Received: 1 August 2018 / Revised: 4 September 2018 / Accepted: 7 September 2018 / Published: 10 September 2018
(This article belongs to the Special Issue Tea in Health and Disease)
The present study investigated the preventive effect of polyphenols in Liubao tea (PLT) on carbon tetrachloride (CCl4)-induced liver injury in mice. The mice were initially treated with PLT, followed by induction of liver injury using 10 mL/kg CCl4. Then liver and serum indices, as well as the expression levels of related messenger RNAs (mRNAs) and proteins in liver tissues were measured. The results showed that PLT reduces the liver quality and indices of mice with liver injury. PLT also downregulates aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides (TGs), and malondialdehyde (MDA), and upregulates superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the sera of mice with liver injury. PLT also reduces serum levels of interleukin-6 (IL-6), interleukin-12 (IL-12), tumor necrosis factor- α (TNF- α ), and interferon- γ (IFN- γ ) cytokines in mice with liver injury. Pathological morphological observation also shows that PLT reduces CCl4-induced central venous differentiation of liver tissues and liver cell damage. Furthermore, qPCR and Western blot also confirm that PLT upregulates the mRNA and protein expressions of Gu/Zn-SOD, Mn-SOD, catalase (CAT), GSH-Px, and nuclear factor of κ -light polypeptide gene enhancer in B-cells inhibitor- α (I κ B- α ) in liver tissues, and downregulates the expression of cyclooxygenase 2 (COX-2) and nuclear factor κ -light-chain-enhancer of activated B cells (NF- κ B). Meanwhile, PLT also raised the phosphorylated (p)-NF- κ B p65 and cytochrome P450 reductase protein expression in liver injury mice. The components of PLT include gallic acid, catechin, caffeine, epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), and epicatechin gallate (ECG), which possibly have a wide range of biological activities. Thus, PLT imparts preventive effects against CCl4-induced liver injury, which is similar to silymarin. View Full-Text
Keywords: polyphenol; Liubao tea; hepatic damage; mRNA expression; protein expression polyphenol; Liubao tea; hepatic damage; mRNA expression; protein expression
Show Figures

Figure 1

MDPI and ACS Style

Pan, Y.; Long, X.; Yi, R.; Zhao, X. Polyphenols in Liubao Tea Can Prevent CCl4-Induced Hepatic Damage in Mice through Its Antioxidant Capacities. Nutrients 2018, 10, 1280.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop