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Nutrients 2018, 10(6), 793;

Inulin Supplementation Does Not Reduce Plasma Trimethylamine N-Oxide Concentrations in Individuals at Risk for Type 2 Diabetes

Department of Human Nutrition, Foods, and Exercise, 295 West Campus Dr., Virginia Tech, Blacksburg, VA 24061, USA
Translational Obesity Research Graduate Education Program, Virginia Tech, Blacksburg, VA 24061, USA
Department of Food Science and Technology, Integrated Life Sciences Building, 1981 Kraft Dr., Blacksburg, VA 24060, USA
The Metabolic Phenotyping Core, 1981 Kraft Dr., Virginia Tech, Blacksburg, VA 24061, USA
Author to whom correspondence should be addressed.
Received: 3 May 2018 / Revised: 8 June 2018 / Accepted: 14 June 2018 / Published: 20 June 2018
(This article belongs to the Special Issue Gut Microbiome and Human Health)
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Trimethylamine N-oxide (TMAO) is associated with type 2 diabetes (T2DM) and increased risk of adverse cardiovascular events. Prebiotic supplementation has been purported to reduce TMAO production, but whether prebiotics reduce fasting or postprandial TMAO levels is unclear. Sedentary, overweight/obese adults at risk for T2DM (n = 18) were randomized to consume a standardized diet (55% carbohydrate, 30% fat) with 10 g/day of either an inulin supplement or maltodextrin placebo for 6 weeks. Blood samples were obtained in the fasting state and hourly during a 4-h high-fat challenge meal (820 kcal; 25% carbohydrate, 63% fat; 317.4 mg choline, 62.5 mg betaine, 8.1 mg l-carnitine) before and after the diet. Plasma TMAO and trimethylamine (TMA) moieties (choline, l-carnitine, betaine, and γ-butyrobetaine) were measured using isocratic ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). There were no differences in fasting or postprandial TMAO or TMA moieties between the inulin and placebo groups at baseline (all p > 0.05). There were no significant changes in fasting or postprandial plasma TMAO or TMA moiety concentrations following inulin or placebo. These findings suggest that inulin supplementation for 6 weeks did not reduce fasting or postprandial TMAO in individuals at risk for T2DM. Future studies are needed to identify efficacious interventions that reduce plasma TMAO concentrations. View Full-Text
Keywords: prediabetes; prebiotic; metabolite; cardiovascular; metabolism prediabetes; prebiotic; metabolite; cardiovascular; metabolism

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Baugh, M.E.; Steele, C.N.; Angiletta, C.J.; Mitchell, C.M.; Neilson, A.P.; Davy, B.M.; Hulver, M.W.; Davy, K.P. Inulin Supplementation Does Not Reduce Plasma Trimethylamine N-Oxide Concentrations in Individuals at Risk for Type 2 Diabetes. Nutrients 2018, 10, 793.

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