Next Article in Journal
FADS Polymorphism, Omega-3 Fatty Acids and Diabetes Risk: A Systematic Review
Next Article in Special Issue
Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content
Previous Article in Journal
Food-Based Social Enterprises and Asylum Seekers: The Food Justice Truck
Previous Article in Special Issue
Milk-Related Symptoms and Immunoglobulin E Reactivity in Swedish Children from Early Life to Adolescence
Open AccessArticle

Immunological Comparison of Native and Recombinant Hen’s Egg Yolk Allergen, Chicken Serum Albumin (Gal d 5), Produced in Kluveromyces lactis

NeuroAllergy Research Laboratory (NARL), School of Life and Environmental Sciences, Faculty of Science, Engineering and Built Environment, Deakin University, 75 Pigdons Road, Geelong, Victoria 3216, Australia
Poultry Cooperative Research Centre, P.O. Box U242, University of New England, Armidale, NSW 2351, Australia
Department of Biological Science, Faculty of Applied Sciences, Rajarata University of Sri Lanka, Mihintale 50300, Sri Lanka
Australian Animal Health Laboratory (AAHL), Biosecurity Flagship, Commonwealth Scientific and Industrial Research Organisation (CSIRO), 5 Portarlington Road, Newcomb, VIC 3219, Australia
Department of Allergy and Immunology, Royal Children’s Hospital, 50 Flemington Road, Parkville, VIC 3052, Australia
Allergy and Immune Disorders, Murdoch Children’s Research Institute, Parkville, Melbourne, Victoria 3052, Australia
Department of Paediatrics, The University of Melbourne, Parkville, Melbourne, Victoria 3052, Australia
Author to whom correspondence should be addressed.
Nutrients 2018, 10(6), 757;
Received: 17 May 2018 / Revised: 5 June 2018 / Accepted: 8 June 2018 / Published: 12 June 2018
(This article belongs to the Special Issue Contributions of Diet and Gastrointestinal Digestion to Food Allergy)
Chicken serum albumin (CSA) is a hen’s egg yolk allergen causing IgE-mediated allergy. The objective of this study was to produce a recombinant version of CSA and compare its IgE reactivity to natural CSA (nCSA). CSA was cloned and expressed as a soluble fraction in the yeast Kluyveromyces lactis (K. lactis) protein expression system. The gene encoding CSA was amplified with a C-terminal hemagglutinin epitope tag by polymerase chain reaction (PCR) and cloned into the pKLAC2 expression vector prior to transforming into K. lactis. Recombinant CSA (rCSA) was purified by immunoprecipitation. Twenty-one patients allergic to hen’s egg white were examined for sensitisation against nCSA. 38% of patients were found to be sensitised to CSA based on Western immunoassay. Immunoglobulin E (IgE) binding capacity of rCSA and nCSA was analysed by ELISA using sera from patients sensitised to CSA. Levels of IgE-binding were similar for both the recombinant and the natural CSA, indicating the existence of similar epitopes. rCSA produced in this study is a potential candidate to be used in component-resolved diagnosis (CRD) of egg yolk allergy. The usefulness of rCSA in CRD of egg yolk allergy warrants further characterisation using sera from patients with allergy to hen’s egg yolk in future studies. View Full-Text
Keywords: allergy; egg yolk allergens; recombinant allergens; chicken serum albumin allergy; egg yolk allergens; recombinant allergens; chicken serum albumin
Show Figures

Figure 1

MDPI and ACS Style

De Silva, C.; Dhanapala, P.; King, S.; Doran, T.; Tang, M.; Suphioglu, C. Immunological Comparison of Native and Recombinant Hen’s Egg Yolk Allergen, Chicken Serum Albumin (Gal d 5), Produced in Kluveromyces lactis. Nutrients 2018, 10, 757.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop