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Open AccessArticle

Anti-Inflammatory and Anti-Apoptotic Effects of Acer Palmatum Thumb. Extract, KIOM-2015EW, in a Hyperosmolar-Stress-Induced In Vitro Dry Eye Model

1
Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu 41062, Korea
2
Institute of Biomedical Engineering Research, Medical School, Kyungpook National University, 90 Chilgokjungang-daero 136-gil, Bukgu, Daegu 41405, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2018, 10(3), 282; https://doi.org/10.3390/nu10030282
Received: 18 January 2018 / Revised: 12 February 2018 / Accepted: 26 February 2018 / Published: 28 February 2018
The aim of this study was to assess the anti-inflammatory and anti-apoptotic effects of KIOM-2015EW, the hot-water extract of maple leaves in hyperosmolar stress (HOS)-induced human corneal epithelial cells (HCECs). HCECs were exposed to hyperosmolar medium and exposed to KIOM-2015EW with or without the hyperosmolar media. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production and apoptosis were observed, and the activation of mitogen-activated protein kinases (MAPKs) including extracellular signal regulated kinase (ERK), p38 and c-JUN N-terminal kinase (JNK) signaling and nuclear factor (NF)-κB was confirmed. Compared to isomolar medium, the induction of cell cytotoxicity significantly increased in HCECs exposed to hyperosmolar medium in a time-dependent manner. KIOM-2015EW-treatment significantly reduced the mRNA and protein expression of pro-inflammatory mediators and apoptosis. KIOM-2015EW-treatment inhibited HOS-induced MAPK signaling activation. Additionally, the HOS-induced increase in NF-κB phosphorylation was attenuated by KIOM-2015EW. The results demonstrated that KIOM-2015EW protects the ocular surface by suppressing inflammation in dry eye disease, and suggest that KIOM-2015EW may be used to treat several ocular surface diseases where inflammation plays a key role. View Full-Text
Keywords: dry eye; human corneal epithelial cells; hyperosmolar stress; anti-inflammation; anti-apoptosis; natural substance; orientin; isoorientin; vitexin dry eye; human corneal epithelial cells; hyperosmolar stress; anti-inflammation; anti-apoptosis; natural substance; orientin; isoorientin; vitexin
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MDPI and ACS Style

Kim, Y.-H.; Oh, T.W.; Park, E.; Yim, N.-H.; Park, K.I.; Cho, W.K.; Ma, J.Y. Anti-Inflammatory and Anti-Apoptotic Effects of Acer Palmatum Thumb. Extract, KIOM-2015EW, in a Hyperosmolar-Stress-Induced In Vitro Dry Eye Model. Nutrients 2018, 10, 282.

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