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Nutrients 2018, 10(3), 276; https://doi.org/10.3390/nu10030276

Cucumis sativus Aqueous Fraction Inhibits Angiotensin II-Induced Inflammation and Oxidative Stress In Vitro

1
Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca, Morelos CP 62209, Mexico
2
Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Leñeros S/N, Cuernavaca, Morelos CP 62350, Mexico
3
Laboratorio de Farmacología, Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Republica de Argentina 1, Xochitepec, Morelos CP 62790, Mexico
4
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Coyoacán, Mexico City CP 04510, Mexico
5
Centro de Investigación en Dinámica Celular (IICBA), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca, Morelos CP 62209, Mexico
6
Departamento de Fisiología Biofísica y Neurociencias del Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV del IPN), Mexico City CP 07360, Mexico
7
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Coyoacán, Mexico City CP 04510, Mexico
*
Author to whom correspondence should be addressed.
Received: 2 February 2018 / Revised: 15 February 2018 / Accepted: 25 February 2018 / Published: 28 February 2018
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Abstract

Inflammation and oxidative stress play major roles in endothelial dysfunction, and are key factors in the progression of cardiovascular diseases. The aim of this study was to evaluate in vitro the effect of three subfractions (SFs) from the Cucumis sativus aqueous fraction to reduce inflammatory factors and oxidative stress induced by angiotensin II (Ang II) in human microvascular endothelial cells-1 (HMEC-1) cells. The cells were cultured with different concentrations of Ang II and 0.08 or 10 μg/mL of SF1, SF2, or SF3, or 10 μmol of losartan as a control. IL-6 (Interleukin 6) concentration was quantified. To identify the most effective SF combinations, HMEC-1 cells were cultured as described above in the presence of four combinations of SF1 and SF3. Then, the effects of the most effective combination on the expression of adhesion molecules, the production of reactive oxygen species (ROS), and the bioavailability of nitric oxide (NO) were evaluated. Finally, a mass spectrometry analysis was performed. Both SF1 and SF3 subfractions decreased the induction of IL-6 by Ang II, and C4 (SF1 and SF3, 10 μg/mL each) was the most effective combination to inhibit the production of IL-6. Additionally, C4 prevented the expression of adhesion molecules, reduced the production of ROS, and increased the bioavailability of NO. Glycine, arginine, asparagine, lysine, and aspartic acid were the main components of both subfractions. These results demonstrate that C4 has anti-inflammatory and antioxidant effects. View Full-Text
Keywords: inflammation; ROS; angiotensin II; Cucumis sativus inflammation; ROS; angiotensin II; Cucumis sativus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Trejo-Moreno, C.; Méndez-Martínez, M.; Zamilpa, A.; Jiménez-Ferrer, E.; Perez-Garcia, M.D.; Medina-Campos, O.N.; Pedraza-Chaverri, J.; Santana, M.A.; Esquivel-Guadarrama, F.R.; Castillo, A.; Cervantes-Torres, J.; Fragoso, G.; Rosas-Salgado, G. Cucumis sativus Aqueous Fraction Inhibits Angiotensin II-Induced Inflammation and Oxidative Stress In Vitro. Nutrients 2018, 10, 276.

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