Transcription Factor Eb Is Required for Macropinocytosis-Mediated Growth Recovery of Nutrient-Deprived Kras-Mutant Cells
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Fluorescence Microscopy
2.3. siRNA Knockdown
2.4. Western Blot Analysis
2.5. Cell Proliferation
2.6. Statistical Analysis
3. Results
3.1. KRAS-Mutant Cells Exhibit Higher Levels of Macropinocytosis Than Kras Wild-Type Cells
3.2. TFEB Promotes Lysosomal Degradation of Extracellular Protein without Affecting Macropinocytotic Uptake
3.3. TFEB Contributes to Macropinocytosis-Mediated Recovery of mTORC1 Activity and Cell Proliferation in Leucine-Deprived KRAS-Mutant Cells
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Jeong, S.; Byun, J.-K.; Cho, S.J.; Chin, J.; Lee, I.-K.; Choi, Y.-K.; Park, K.-G. Transcription Factor Eb Is Required for Macropinocytosis-Mediated Growth Recovery of Nutrient-Deprived Kras-Mutant Cells. Nutrients 2018, 10, 1638. https://doi.org/10.3390/nu10111638
Jeong S, Byun J-K, Cho SJ, Chin J, Lee I-K, Choi Y-K, Park K-G. Transcription Factor Eb Is Required for Macropinocytosis-Mediated Growth Recovery of Nutrient-Deprived Kras-Mutant Cells. Nutrients. 2018; 10(11):1638. https://doi.org/10.3390/nu10111638
Chicago/Turabian StyleJeong, Seokmin, Jun-Kyu Byun, Sung Jin Cho, Jungwook Chin, In-Kyu Lee, Yeon-Kyung Choi, and Keun-Gyu Park. 2018. "Transcription Factor Eb Is Required for Macropinocytosis-Mediated Growth Recovery of Nutrient-Deprived Kras-Mutant Cells" Nutrients 10, no. 11: 1638. https://doi.org/10.3390/nu10111638
APA StyleJeong, S., Byun, J.-K., Cho, S. J., Chin, J., Lee, I.-K., Choi, Y.-K., & Park, K.-G. (2018). Transcription Factor Eb Is Required for Macropinocytosis-Mediated Growth Recovery of Nutrient-Deprived Kras-Mutant Cells. Nutrients, 10(11), 1638. https://doi.org/10.3390/nu10111638