Next Article in Journal
Ingestion of Insect Protein Isolate Enhances Blood Amino Acid Concentrations Similar to Soy Protein in A Human Trial
Next Article in Special Issue
Purification and Identification of Angiotensin I-Converting Enzyme Inhibitory Peptides and the Antihypertensive Effect of Chlorella sorokiniana Protein Hydrolysates
Previous Article in Journal
Human Breast Milk NMR Metabolomic Profile across Specific Geographical Locations and Its Association with the Milk Microbiota
Previous Article in Special Issue
Hairless Canaryseed: A Novel Cereal with Health Promoting Potential
Open AccessArticle

A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate

1
Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University, 1-18-1 Kaga, Itabashi-ku, Tokyo 173-8602, Japan
2
Q’sai Co., Ltd., 1-7-16 Kusagae, Chuo-ku Fukuoka City 810-8606, Japan
3
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-Cho, Kyoto 606-8502, Japan
*
Author to whom correspondence should be addressed.
Nutrients 2018, 10(10), 1356; https://doi.org/10.3390/nu10101356
Received: 1 September 2018 / Revised: 17 September 2018 / Accepted: 20 September 2018 / Published: 22 September 2018
(This article belongs to the Special Issue Impact of Bioactive Peptides on Human Health)
Levels of short linear hydroxyproline (Hyp)-containing peptides, such as prolyl-hydroxyproline (Pro-Hyp), increase in human blood after the ingestion of collagen hydrolysate, which has been associated with beneficial effects for human skin and joints. The present study demonstrates the presence of a novel food-derived collagen peptide, cyclic Pro-Hyp, in human blood after the ingestion of collagen hydrolysate. The cyclic Pro-Hyp levels in plasma samples were estimated by liquid chromatography mass spectrometry (LC-MS). Cyclic Pro-Hyp levels significantly increased in the plasma after ingestion of collagen hydrolysate, reaching a maximum level after 2 h and then decreasing. The maximum level of cyclic Pro-Hyp in plasma ranged from 0.1413 to 0.3443 nmol/mL, representing approximately 5% of linear Pro-Hyp in plasma after ingestion of collagen hydrolysate. Addition of cyclic Pro-Hyp in medium at 7 nmol/mL significantly enhanced the growth rate of mouse skin fibroblasts on collagen gel more extensively compared to linear Pro-Hyp. View Full-Text
Keywords: cyclic Pro-Hy; Pro-Hyp; collagen hydrolysate; collagen peptides; fibroblasts; mouse skin cyclic Pro-Hy; Pro-Hyp; collagen hydrolysate; collagen peptides; fibroblasts; mouse skin
Show Figures

Figure 1

MDPI and ACS Style

Shigemura, Y.; Iwasaki, Y.; Tateno, M.; Suzuki, A.; Kurokawa, M.; Sato, Y.; Sato, K. A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate. Nutrients 2018, 10, 1356. https://doi.org/10.3390/nu10101356

AMA Style

Shigemura Y, Iwasaki Y, Tateno M, Suzuki A, Kurokawa M, Sato Y, Sato K. A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate. Nutrients. 2018; 10(10):1356. https://doi.org/10.3390/nu10101356

Chicago/Turabian Style

Shigemura, Yasutaka; Iwasaki, Yu; Tateno, Mana; Suzuki, Asahi; Kurokawa, Mihoko; Sato, Yoshio; Sato, Kenji. 2018. "A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate" Nutrients 10, no. 10: 1356. https://doi.org/10.3390/nu10101356

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop