A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collagen Hydrolysate
2.2. Chemicals
2.3. Preparation of Cyclic Pro-Hyp
2.4. Human Study
2.5. Separation of Linear and Cyclic Pro-Hyp from Human Plasma by Strong-Cation Exchange Resin
2.6. Estimation of Hyp-Containing Peptide Concentration
2.7. Liquid Chromatograph–Mass Spectrometer (LC-MS) Analysis
2.8. Cell Culture
2.9. Statistical Analysis
3. Results
3.1. Preparation of Cyclic Pro-Hyp
3.2. Hyp Concentration in Plasma Cyclic Peptide Fractions
3.3. Changes in the Concentration of Cyclic Pro-Hyp in Human Plasma
3.4. Cell Culture
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Shigemura, Y.; Iwasaki, Y.; Tateno, M.; Suzuki, A.; Kurokawa, M.; Sato, Y.; Sato, K. A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate. Nutrients 2018, 10, 1356. https://doi.org/10.3390/nu10101356
Shigemura Y, Iwasaki Y, Tateno M, Suzuki A, Kurokawa M, Sato Y, Sato K. A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate. Nutrients. 2018; 10(10):1356. https://doi.org/10.3390/nu10101356
Chicago/Turabian StyleShigemura, Yasutaka, Yu Iwasaki, Mana Tateno, Asahi Suzuki, Mihoko Kurokawa, Yoshio Sato, and Kenji Sato. 2018. "A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate" Nutrients 10, no. 10: 1356. https://doi.org/10.3390/nu10101356
APA StyleShigemura, Y., Iwasaki, Y., Tateno, M., Suzuki, A., Kurokawa, M., Sato, Y., & Sato, K. (2018). A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate. Nutrients, 10(10), 1356. https://doi.org/10.3390/nu10101356