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Open AccessArticle

Analysis of Cold-Developed vs. Cold-Acclimated Leaves Reveals Various Strategies of Cold Acclimation of Field Pea Cultivars

1
Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Biophysics, Faculty of Science, Palacký University, 783 71 Olomouc, Czech Republic
2
Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Chemical Biology and Genetics, Faculty of Science, Palacký University, 783 71 Olomouc, Czech Republic
3
Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, 783 71 Olomouc, Czech Republic
*
Author to whom correspondence should be addressed.
Remote Sens. 2019, 11(24), 2964; https://doi.org/10.3390/rs11242964
Received: 1 November 2019 / Revised: 2 December 2019 / Accepted: 6 December 2019 / Published: 11 December 2019
(This article belongs to the Special Issue Advanced Imaging for Plant Phenotyping)
Peas (Pisum sativum L.) belong among the world’s oldest domesticated crops, serving as a source of proteins, complex carbohydrates, vitamins and minerals. Autumn sowing allows a higher biomass production as well as the avoidance of the drought and heat stresses of late spring. However, the character of European continental winters limits plant growth and development through cold stress. This work sought parameters that reflect the cold tolerance of pea plants and consequently to suggest an afila-type pea cultivar with resilience to European continental winters. For this purpose, we employed indoor remote sensing technology and compared the 22-day-long acclimation to 5 °C of four pea cultivars: Arkta, with normal leaves and the known highest cold resistance to European continental winters, and Enduro, Terno and CDC Le Roy, all of the afila type. Besides evaluation of shoot growth rate and quenching analysis of chlorophyll fluorescence (ChlF) by imaging methods, we measured the chlorophyll content and ChlF induction with a nonimaging fluorometer. Here we show that the acclimation to cold of the Arkta exhibits a different pattern than the other cultivars. Arkta showed the fastest retardation of photosynthesis and shoot growth, which might be part of its winter survival strategy. Terno, on the other hand, showed sustained photosynthetic performance and growth, which might be an advantageous strategy for spring. Surprisingly, Enduro showed sustained photosynthesis in the stipules, which transferred and acclimated to 5 °C (cold-acclimated). However, of all the cultivars, Enduro had the strongest inhibition of photosynthesis in new stipules that developed after the transition to cold (cold-developed). We conclude that the parameters of ChlF spatial imaging calculated as averages from whole plants are suboptimal for the characterization of various cold acclimation strategies. The most marked changes were obtained when the new cold-developed leaves were analyzed separately from the rest of the plant. View Full-Text
Keywords: Pea (Pisum); cold acclimation; cold-developed; plant phenotyping; chlorophyll fluorescence; photochemistry of photosystem II Pea (Pisum); cold acclimation; cold-developed; plant phenotyping; chlorophyll fluorescence; photochemistry of photosystem II
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Husičková, A.; Humplík, J.F.; Hýbl, M.; Spíchal, L.; Lazár, D. Analysis of Cold-Developed vs. Cold-Acclimated Leaves Reveals Various Strategies of Cold Acclimation of Field Pea Cultivars. Remote Sens. 2019, 11, 2964.

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