Imbibition and Germination of Seeds with Economic and Ecological Interest: Physical and Biochemical Factors Involved
Round 1
Reviewer 1 Report
Thank you for the opportunity to review the manuscript ” Imbibition and Germination of Seeds with Economic and Ecological Interest: Physical and Biochemical Factors Involved” aimed at proving new research inputs regarding the water imbibition speed and electrical leakage during imbibition to test the seed quality and describe the role of water imbibition to promote the respiration rate in fresh and imbibed seeds and evaluate the role of electrical leakage as a good test to evaluate seed viability which end with seed germination.
The article made a positive impression on me. It is clear that the authors have done a great job. However, I miss the review of the theory – I couldnt understand why it is worthy and how sustainability can benefit from this new knowledge. International works on this issue should be studied more and incorporated into the discussion section. Also strengthen the link with the circular economy. Based on these comments, the article can be printed.
Author Response
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Best regards
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Experiments are relevant and included different species. However, it is recommended to add some schematic diagram of the related mechanisms to improve the readability of the manuscript.
Author Response
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Best regards
Author Response File: Author Response.pdf
Reviewer 3 Report
The paper presents original data related to imbibition assays in 14 plant species. The number of plant species could be increased; at least 2 different species per family could be inserted the paper.
please give the genus name in binominal taxonomical names in where it first time to be passed (e.g. Arachis hypogaea L. instead of A. hypogaea L.)
Please edit table 1 as Table 1 in text.
the paper is missing transcript and/or pretin/metabolite profiling which could support the findings of the study.
Please give details for R statistics instead of giving the package name (GerminaR)
Is dendogram icluding at least two dimentional findings? It is not clear in materials and methods section. Correlation matrix should be carried out to evaluate the different experimental data obtained in dendogram. Or, component analysis could be added to the paper.
Author Response
Please verify the attached letter.
Best regards
Author Response File: Author Response.pdf
Round 2
Reviewer 3 Report
I'd like to thank authors to edit the paper according to the recommendations.
I hope the authors could produce a new project including more plant species and protein/metabolite profiling in near future.
