Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Soil Sample Collection
2.3. Determination of Soil Physical and Chemical Properties
2.4. Soil Microbial Illumina Sequencing
2.5. Data Processing Analysis
3. Results
3.1. Soil Physicochemical Properties
3.2. Composition of Soil Microbial Community
3.3. Richness and Diversity of Soil Microbial Community
3.4. Structure of Soil Microbial Community
3.5. Relationship between Soil Microbial Community and Physicochemical Properties
3.6. Predicted Functional Analysis for Microbial Community
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Samples | pH | TOC (g/kg) | TN (g/kg) | TP (g/kg) | NH4+-N (mg/kg) | NO3−-N (mg/kg) | SWC (%) |
---|---|---|---|---|---|---|---|
BP20 | 4.99 ± 0.07 b | 57.86 ± 5.45 a | 3.45 ± 0.35 a | 1.39 ± 0.25 a | 8.27 ± 0.49 b | 12.00 ± 2.49 a | 30.87 ± 3.04 a |
BP40 | 5.17 ± 0.11 a | 31.47 ± 4.37 b | 2.72 ± 0.26 b | 0.73 ± 0.13 b | 11.16 ± 1.27 a | 8.04 ± 0.93 b | 36.21 ± 5.32 a |
BP60 | 5.04 ± 0.03 b | 16.15 ± 2.61 c | 1.41 ± 0.12 c | 0.47 ± 0.32 b | 12.16 ± 2.18 a | 7.31 ± 1.08 b | 21.44 ± 1.40 b |
LG20 | 5.67 ± 0.14 ab | 39.27 ± 4.32 a | 3.07 ± 0.43 a | 1.80 ± 0.33 a | 8.43 ± 0.35 b | 5.73 ± 0.35 a | 39.10 ± 4.69 a |
LG40 | 5.79 ± 0.06 a | 20.01 ± 2.08 b | 2.23 ± 0.39 b | 0.61 ± 0.48 b | 10.99 ± 1.53 a | 3.48 ± 0.73 b | 32.50 ± 6.57 a |
LG60 | 5.54 ± 0.08 b | 15.20 ± 3.63 b | 1.09 ± 0.17 c | 0.39 ± 0.11 b | 11.31 ± 2.76 a | 3.41 ± 0.52 b | 23.23 ± 2.46 b |
Samples | Shannon-Wiener | Simpson | ACE | Chao1 |
---|---|---|---|---|
BP20 | 5.49 ± 0.08 a | 0.013 ± 0.004 a | 1328.35 ± 37.48 a | 1305.26 ± 38.52 a |
BP40 | 5.12 ± 0.21 b | 0.015 ± 0.003 a | 1295.32 ± 53.04 a | 1288.06 ± 65.80 a |
BP60 | 5.15 ± 0.16 b | 0.014 ± 0.002 a | 1343.42 ± 46.35 a | 1344.03 ± 47.65 a |
LG20 | 5.42 ± 0.07 b | 0.010 ± 0.003 b | 1452.31 ± 50.18 a | 1417.03 ± 49.59 a |
LG40 | 5.07 ± 0.24 c | 0.016 ± 0.002 a | 1325.23 ± 23.41 b | 1309.08 ± 39.42 b |
LG60 | 5.78 ± 0.25 a | 0.008 ± 0.004 b | 1413.24 ± 33.86 a | 1390.13 ± 26.58 a |
Factor | Forest Type | Depth | Forest Type × Depth | |||
---|---|---|---|---|---|---|
F | p | F | p | F | p | |
Shannon | 3.84 | 0.074 | 7.72 | 0.007 | 6.82 | 0.010 |
Simpson | 3.03 | 0.108 | 4.84 | 0.029 | 1.88 | 0.195 |
ACE | 14.19 | 0.003 | 6.34 | 0.013 | 1.90 | 0.019 |
Chao1 | 7.50 | 0.018 | 4.05 | 0.045 | 1.54 | 0.025 |
Phyla | 26.27 | <0.001 | 7.15 | 0.025 | 3.10 | 0.028 |
Genera | 18.24 | <0.001 | 5.52 | 0.041 | 4.15 | 0.022 |
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Song, D.; Cui, Y.; Ma, D.; Li, X.; Liu, L. Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains. Sustainability 2022, 14, 9284. https://doi.org/10.3390/su14159284
Song D, Cui Y, Ma D, Li X, Liu L. Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains. Sustainability. 2022; 14(15):9284. https://doi.org/10.3390/su14159284
Chicago/Turabian StyleSong, Dandan, Yuanquan Cui, Dalong Ma, Xin Li, and Lin Liu. 2022. "Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains" Sustainability 14, no. 15: 9284. https://doi.org/10.3390/su14159284