A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Characteristics of the Consortia and HCs
2.2. Methanogenic Tests
2.3. Analytical Methods
3. Results
3.1. Influence of HCs on the ATP Level of Cells in the Natural Anaerobic Consortia Catalyzing Methanogenesis
3.2. Analysis of the Biogas Production Efficiency and CH4 Content in Biogas Accumulated by Methanogenic Consortia in the Presence of Different Concentrations of HCs
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Consortium | Dry Matter (g/L) | Ash (%) | Biomass VSS (g/L) | * Activity (mg COD/g VSS/day) | Initial Concentration of ATP, ×10−12 mol/mL | |
---|---|---|---|---|---|---|
Acidogenic | Methanogenic | |||||
I | 64.3 ± 3.1 | 42.4 ± 0.9 | 35.3 ± 1.1 | 2210 ± 30 | 315 ± 5 | 15.7 ± 0.6 |
II | 57.1 ± 2.3 | 37.8 ± 1.6 | 35.4 ± 1.2 | 1550 ± 30 | 86 ± 1 | 26.4 ± 0.8 |
III | 56.6 ± 1.6 | 38.5 ± 1.5 | 34.8 ± 1.1 | 2180 ± 30 | 195 ± 10 | 4.4 ± 0.3 |
IV | 58.2 ± 2.2 | 42.7 ± 1.7 | 33.0 ± 1.1 | 1970 ± 30 | 157 ± 1 | 15.1 ± 0.6 |
HС, g/L | Consortium | |||
---|---|---|---|---|
I | II | III | IV | |
0 (control) | 14.5 ± 0.5 | 6.6 ± 0.3 | 6.3 ± 0.3 | 10.2 ± 0.4 |
PH | ||||
1 | 12.7 ± 0.4 | 5.2 ± 0.2 | 0.7 ± 0.1 | 1.5 ± 0.2 |
5 | 4.5 ± 0.3 | 1.7 ± 0.2 | 0.3 ± 0.1 | 0.9 ± 0.1 |
10 | 0.5 ± 0.1 | 0.9 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.1 |
FA | ||||
1 | 20.1 ± 0.6 | 5.9 ± 0.3 | 25.2 ± 0.6 | 2.3 ± 0.2 |
5 | 24.9 ± 0.7 | 3.5 ± 0.2 | 17.6 ± 0.5 | 1.5 ± 0.2 |
10 | 32.9 ± 0.9 | 2.1 ± 0.2 | 15.4 ± 0.4 | 0.6 ± 0.1 |
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Stepanov, N.; Senko, O.; Perminova, I.; Efremenko, E. A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis. Sustainability 2019, 11, 3158. https://doi.org/10.3390/su11113158
Stepanov N, Senko O, Perminova I, Efremenko E. A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis. Sustainability. 2019; 11(11):3158. https://doi.org/10.3390/su11113158
Chicago/Turabian StyleStepanov, Nikolay, Olga Senko, Irina Perminova, and Elena Efremenko. 2019. "A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis" Sustainability 11, no. 11: 3158. https://doi.org/10.3390/su11113158