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Sustainability 2018, 10(7), 2433;

Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste

GICOM Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Edifici Q, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
Aeris Tecnologías Ambientales S.L., Carrer Santa Rosa, 38, local, 08290 Cerdanyola del Vallès, Barcelona, Spain
Author to whom correspondence should be addressed.
Received: 15 June 2018 / Revised: 6 July 2018 / Accepted: 7 July 2018 / Published: 12 July 2018
(This article belongs to the Special Issue The New Paradigm of Waste Management: Waste as Resources)
PDF [1940 KB, uploaded 12 July 2018]


Solid-state fermentation (SSF) is a promising technology for producing bioproducts from organic wastes. The objective of this study is to assess the feasibility of using digestate as substrate to produce hydrolytic enzymes, mainly cellulase and xylanase, by exploring three different inoculation strategies: (i) SSF with autochthonous microbiota; (ii) non-sterile SSF inoculated with Trichoderma reesei and (iii) sequential batch operation to select a specialized inoculum, testing two different residence times. Native microbial population did not show a significant cellulase production, suggesting the need for a specialized inoculum. The inoculation of Trichoderma reesei did not improve the enzymatic activity. On the other hand, inconsistent operation was achieved during sequential batch reactor in terms of specific oxygen uptake rate, temperature and enzymatic activity profile. Low cellulase and xylanase activities were attained and the main hypotheses are non-appropriate biomass selection and some degree of hydrolysis by non-targeted proteases produced during fermentation. View Full-Text
Keywords: cellulase; digestate; solid-state fermentation; specialized inoculum; xylanase cellulase; digestate; solid-state fermentation; specialized inoculum; xylanase

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Mejias, L.; Cerda, A.; Barrena, R.; Gea, T.; Sánchez, A. Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste. Sustainability 2018, 10, 2433.

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