Cattle Diets Strongly Affect Nitrous Oxide in the Rumen
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals, Diets, and Experimental Design
2.2. Sampling and Measurements of Gaseous Emissions
2.3. Laboratory Analyses of the Forages
- Alfalfa silage [32]:DMcor = DM + (1.05 − 0.059 × pH) × total volatile fatty acids (VFA, C2 − C6) + 0.08 × lactic acid + 0.77 × 1,2-propanediol + 0.87 × 2,3-butanediol + 1.00 × total of other alcohols.
- Corn silage [33]:DMcor = DM + 0.95 × VFA (C2 − C6) + 0.08 × lactic acid + 0.77 × 1,2-propanediol + 1.00 × other alcohols.
- Corn silage [36]:ME = 0.136 × GP + 0.0057 × CP + 0.000286 × EE2 + 2.20.
- Alfalfa silage [37]:ME [MJ/kg organic matter] = 11.09 − 0.01040 × ADFom + 0.00497 × CP + 0.00750 × EE + 0.0351 × GP; ME [MJ/kg DM] = ME (MJ/kg organic matter) × [1000 − ash (g/kg DM)]/1000.
- Grass hay [38]:ME = 7.81 + 0.07559 × GP + 0.00384 × ash + 0.00565 × CP + 0.01898 × EE − 0.00831 × ADFom.
2.4. Statistical Analyses
3. Results
3.1. Gas Production from Forages
3.2. Gas Composition in the Rumen
4. Discussion
4.1. Emissions from Forages
4.2. Concentration Ratios in the Rumen
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Corn Silage (CS) | Alfalfa Silage (AS) | Grass Hay (GH) | |
---|---|---|---|
DM [g/kg] | 366 | 415 | 881 |
Ash | 34.9 | 124 | 70.5 |
Crude protein | 70.7 | 246 | 79.2 |
Ether extract | 35.9 | 30.2 | 20.2 |
aNDFom 1 | 314 | 396 | 599 |
ADFom 2 | 175 | 300 | 340 |
Acid detergent lignin | 17.4 | 98.3 | 36.8 |
Starch | 438 | n.a. | n.a. |
In vitro gas production [mL/200 mg DM] | 64.1 | 39.8 | 50.5 |
Metabolizable energy [MJ/kg DM] | 11.7 | 8.78 | 9.40 |
pH | 3.9 | 5.77 | n.a. |
Lactic acid | 40.7 | 8.2 | n.a. |
Acetic acid | 9.9 | 6.3 | n.a. |
Butyric acid | n.d. 3 | n.d. | n.a. |
Methanol | 0.3 | 1.5 | n.a. |
Ethanol | 1.7 | 1.6 | n.a. |
Water-soluble carbohydrates | 13.4 | 49.8 | n.a. |
NH3-N [g/kg total N] | 109 | 96.7 | n.a. |
Ethyl acetate [mg/kg DM] | 54.4 | 19.3 | n.a. |
Ethyl lactate [mg/kg DM] | 105 | n.d. | n.a. |
Least Square Means | Effect | |||||||
---|---|---|---|---|---|---|---|---|
CS | AS | GH | SEM | F | P | F·P | ||
N2O [μg/(kg dry matter × h)] | 8 a.m. | 0.109 b | 24.1 a | 0.233 b | 3.81 | 0.02 | n.s. | n.s. |
11 a.m. | 0.140 b | 2.46 a | 0.176 b | 0.172 | <0.01 | 0.01 | <0.01 | |
CO2 [mg/(kg dry matter × h)] | 8 a.m. | 391 a | 141 b | 8.13 c | 32.0 | <0.01 | n.s. | n.s. |
11 a.m. | 170 a | 19.0 b | 9.38 b | 14.4 | <0.01 | n.s. | n.s. |
Least Square Means | Effect | ||||||
---|---|---|---|---|---|---|---|
Corn Silage (CS) | Alfalfa Silage (AS) | Grass Hay (GH) | SEM | F | P | F·P | |
DMI [kg/180 min] | 4.60 b | 3.22 b | 6.64 a | 0.473 | <0.01 | 0.02 | <0.01 |
N2O [ppm] | 0.246 b | 0.857 a | 0.171 b | 0.068 | <0.01 | n.s. | 0.02 |
CH4 [%] | 16.9 b | 20.6 a | 20.3 a | 0.890 | 0.01 | <0.01 | 0.03 |
CO2 [%] | 46.1 b | 41.1 b | 54.8 a | 2.21 | <0.01 | n.s. | 0.03 |
CH4:CO2 | 0.358 b | 0.501 a | 0.372 b | 0.010 | <0.01 | 0.02 | <0.01 |
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Gerlach, K.; Schmithausen, A.J.; Sommer, A.C.H.; Trimborn, M.; Büscher, W.; Südekum, K.-H. Cattle Diets Strongly Affect Nitrous Oxide in the Rumen. Sustainability 2018, 10, 3679. https://doi.org/10.3390/su10103679
Gerlach K, Schmithausen AJ, Sommer ACH, Trimborn M, Büscher W, Südekum K-H. Cattle Diets Strongly Affect Nitrous Oxide in the Rumen. Sustainability. 2018; 10(10):3679. https://doi.org/10.3390/su10103679
Chicago/Turabian StyleGerlach, Katrin, Alexander J. Schmithausen, Ansgar C. H. Sommer, Manfred Trimborn, Wolfgang Büscher, and Karl-Heinz Südekum. 2018. "Cattle Diets Strongly Affect Nitrous Oxide in the Rumen" Sustainability 10, no. 10: 3679. https://doi.org/10.3390/su10103679