Hb Mazandaran (α1) α51 Gly > Cys(CE9), c.154 GGC > TGC: A Novel Haemoglobin Variant of α1-Globin Gene
Round 1
Reviewer 1 Report
The mutation identified has lead toa changed amino acid, if comment would have explained any tertiary or quarternary structure, it would have been very appropriate
Author Response
Dear Reviewer
Thanks for your comments.
Unfortunately, it is not possible for us to study the effect of the amino acid change on the structure of the protein. Moreover, We do not access the case for resampling.
Regards
Reviewer 2 Report
I have some comments that I think can improve the manuscript:
1)In table 1 add hemoglobin capillary electrophoresis indices (Hb A2%, Hb F%, Hb Mazandaran%).
2) Hb Mazandaran is a new Hb. Three different variants of Hb have been identified at codon 52, but amino acid change is different. Utilizing a program (for example PolyPhen-2 http://genetics.bwh.harvard.edu/pph2/), the authors can predict the potential effects of amino acid substitution on protein function.
Author Response
Dear Reviewer
Thanks for your helpful comments, The Article is changed based on your comments and the requested parts are presented in the text.
Regards