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Microbiology Research
Volume 13
Issue 2
10.3390/microbiolres13020026
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Article

Two Screening Assays to Detect Vancomycin-Resistant Enterococcus spp.

by
Beniamino T. Cenci-Goga
1,2,*,
Musafiri Karama
2,
Saeed El-Ashram
3,4,
Cristina Saraiva
5,6,
Juan García-Díez
5,
Athanasios Chalias
7 and
Luca Grispoldi
1
1
Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, 06126 Perugia, Italy
2
Faculty of Veterinary Science, Department of Paraclinical Sciences, University of Pretoria, Onderstepoort 0110, South Africa
3
School of Life Science and Engineering, Foshan University, Foshan 528231, China
4
Faculty of Science, Kafrelsheikh University, Kafr el-Sheikh 33516, Egypt
5
Veterinary and Animal Research Centre (CECAV), University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal
6
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal
7
European Food Safety Authority, EU-FORA Programme, 43126 Parma, Italy
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2022, 13(2), 332-341; https://doi.org/10.3390/microbiolres13020026
Submission received: 25 April 2022 / Revised: 13 May 2022 / Accepted: 8 June 2022 / Published: 13 June 2022
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Abstract

Enterococci have become major nosocomial pathogens. An increasing number of these infections are as a result of vancomycin-resistant enterococci. Accurate detection of vancomycin-resistant enterococci (VRE) is important, so that appropriate therapy and infection control measures may be instituted, including veterinary surveillance. Two screening assays to detect vancomycin resistance in enterococci are proposed. Barnes Basal Medium agar (Ba) and Brain Heart Infusion (BHI) broth (plus 1% TTC-2,3,5-triphenyltetrazolium chloride) with several concentrations of vancomycin were used in this work. Five Enterococcus casseliflavus strains with low-level resistance to vancomycin (4 µg/mL) were used. Both media were able to quickly detect the breakpoint of the vancomycin-resistant strains used in this work, and also provided insight into the dynamics of the antibiotic effect at a low concentration on the tested bacterial suspensions.
Keywords: enterococcus; vancomycin; antibiotic resistance; screening enterococcus; vancomycin; antibiotic resistance; screening
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MDPI and ACS Style

Cenci-Goga, B.T.; Karama, M.; El-Ashram, S.; Saraiva, C.; García-Díez, J.; Chalias, A.; Grispoldi, L. Two Screening Assays to Detect Vancomycin-Resistant Enterococcus spp. Microbiol. Res. 2022, 13, 332-341. https://doi.org/10.3390/microbiolres13020026

AMA Style

Cenci-Goga BT, Karama M, El-Ashram S, Saraiva C, García-Díez J, Chalias A, Grispoldi L. Two Screening Assays to Detect Vancomycin-Resistant Enterococcus spp. Microbiology Research. 2022; 13(2):332-341. https://doi.org/10.3390/microbiolres13020026

Chicago/Turabian Style

Cenci-Goga, Beniamino T., Musafiri Karama, Saeed El-Ashram, Cristina Saraiva, Juan García-Díez, Athanasios Chalias, and Luca Grispoldi. 2022. "Two Screening Assays to Detect Vancomycin-Resistant Enterococcus spp." Microbiology Research 13, no. 2: 332-341. https://doi.org/10.3390/microbiolres13020026

APA Style

Cenci-Goga, B. T., Karama, M., El-Ashram, S., Saraiva, C., García-Díez, J., Chalias, A., & Grispoldi, L. (2022). Two Screening Assays to Detect Vancomycin-Resistant Enterococcus spp. Microbiology Research, 13(2), 332-341. https://doi.org/10.3390/microbiolres13020026

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