Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods
Abstract
:1. Introduction
2. Materials and Methods
2.1. Botanical Material
2.2. Chromatographic Analysis
2.3. Solubilization of the Compound for Tests of Biological Activity
2.4. Cultivation of Human Cell Lines
2.5. Cytotoxicity Assays
2.6. Assessment of Hemolytic Activity
2.7. In Vitro Culture of Plasmodium spp.
2.8. In Vitro Culture of Intraerythrocytic Stages of Plasmodium falciparum
2.9. Determination of Parasitemia
2.10. Synchronization of Plasmodium falciparum Cultivation
2.11. In Vitro Schizonticidal Testing with Plasmodium falciparum
2.12. Selectivity Index
2.13. Statistical Analysis
3. Results
3.1. Chromatographic Analysis
3.2. Antiplasmodial Activity and Cytotoxicity
3.3. Hemolytic Activity
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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rt (min) | Compound | Rel.% |
---|---|---|
4.28 | Gallic acid | 14.75 |
6.05 | Gallotannin | 15.19 |
6.8 | Valoneic acid dilactone | 13.89 |
7.68 | Ellagic acid | 34.27 |
TOTAL: | 78.1 |
Test Substance | IC50 (µg/mL) ± SD | ||
---|---|---|---|
P. f. (W2) | WI-26-VA4 | SI | |
Jucá hydroalcoholic extract | 11.10 ± 1.13 | >100 | 9 |
Chloroquine | 0.21 ± 0.13 | >100 | 476 |
Artemether | 0.01 ± 0.28 | >100 | 10000 |
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Assis, F.F.V.d.; Almeida Junior, J.S.d.; Moraes, T.M.P.; Varotti, F.d.P.; Moraes, C.C.; Sartoratto, A.; Moraes, W.P.; Minervino, A.H.H. Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods. Pharmaceutics 2023, 15, 1162. https://doi.org/10.3390/pharmaceutics15041162
Assis FFVd, Almeida Junior JSd, Moraes TMP, Varotti FdP, Moraes CC, Sartoratto A, Moraes WP, Minervino AHH. Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods. Pharmaceutics. 2023; 15(4):1162. https://doi.org/10.3390/pharmaceutics15041162
Chicago/Turabian StyleAssis, Francisco Flávio Vieira de, José Sousa de Almeida Junior, Tânia Mara Pires Moraes, Fernando de Pilla Varotti, Camila Castilho Moraes, Adilson Sartoratto, Waldiney Pires Moraes, and Antonio Humberto Hamad Minervino. 2023. "Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods" Pharmaceutics 15, no. 4: 1162. https://doi.org/10.3390/pharmaceutics15041162
APA StyleAssis, F. F. V. d., Almeida Junior, J. S. d., Moraes, T. M. P., Varotti, F. d. P., Moraes, C. C., Sartoratto, A., Moraes, W. P., & Minervino, A. H. H. (2023). Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods. Pharmaceutics, 15(4), 1162. https://doi.org/10.3390/pharmaceutics15041162