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Open AccessArticle

Satureja montana L. Essential Oils: Chemical Profiles/Phytochemical Screening, Antimicrobial Activity and O/W NanoEmulsion Formulations

1
Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma-Piazzale Aldo Moro 5, 00185 Roma, Italy
2
Dipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Roma, Italy
3
National Center of Innovative Technologies in Public Health, Italian National Institute of Health, Viale Regina Elena 299, 00161 Rome, Italy
4
Dipartimento di Farmacia, Università G. d’Annunzio Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
5
Center for Life Nano [email protected], Fondazione Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161 Rome, Italy
*
Author to whom correspondence should be addressed.
These equally contributed to the work.
Pharmaceutics 2020, 12(1), 7; https://doi.org/10.3390/pharmaceutics12010007
Received: 8 October 2019 / Revised: 6 December 2019 / Accepted: 12 December 2019 / Published: 19 December 2019
(This article belongs to the Special Issue Emergent Strategies for Natural Products Delivery)
Chemical fingerprints of four different Satureja montana L. essential oils (SEOs) were assayed by an untargeted metabolomics approach based on Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) coupled with either electrospray ionization or atmospheric pressure chemical ionization ion sources. Analysis and relative quantification of the non-polar volatile fraction were conducted by gas chromatography (GC) coupled to MS. FT-ICR MS confirmed significant differences in the polar metabolite composition, while GC-MS analyses confirmed slight fluctuations in the relative amount of major terpenes and terpenoids, known to play a key role in antimicrobial mechanisms. Oil in eater (O/W) nanoemulsions (NEs) composed by SEOs and Tween 20 or Tween 80 were prepared and analyzed in terms of hydrodynamic diameter, ζ-potential and polydispersity index. The results confirm the formation of stable NEs homogeneous in size. Minimum inhibitory and minimum bactericidal concentrations of SEOs were determined towards Gram-positive (Listeria monocytogenes, Staphylococcus aureus, Staphylococcus haemolyticus) and Gram-negative clinical isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Serratia marcescens). Commercial SEO showed strongest antibacterial activity, while SEO 3 was found to be the most active among the lab made extractions. MIC and MBC values ranged from 0.39 to 6.25 mg·mL−1. Furthermore, a SEO structured in NEs formulation was able to preserve and improve antimicrobial activity. View Full-Text
Keywords: Satureja montana L.; essential oils; nanoformulation; nanoemulsions; antibacterial activity; FT-ICR mass spectrometry Satureja montana L.; essential oils; nanoformulation; nanoemulsions; antibacterial activity; FT-ICR mass spectrometry
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Maccelli, A.; Vitanza, L.; Imbriano, A.; Fraschetti, C.; Filippi, A.; Goldoni, P.; Maurizi, L.; Ammendolia, M.G.; Crestoni, M.E.; Fornarini, S.; Menghini, L.; Carafa, M.; Marianecci, C.; Longhi, C.; Rinaldi, F. Satureja montana L. Essential Oils: Chemical Profiles/Phytochemical Screening, Antimicrobial Activity and O/W NanoEmulsion Formulations. Pharmaceutics 2020, 12, 7.

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