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Open AccessArticle

Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties

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Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei körút 98, H-4032 Debrecen, Hungary
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ICBMS, UMR 5246, Université Lyon 1, F69622 Villeurbanne, France
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LMI CNRS UMR 5615, Université Lyon 1, 69622 Villeurbanne, France
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LIMMS/CNRS-IIS UMI 2820, Institute of Industrial Science, The University of Tokyo, Tokyo 153-8505, Japan
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Department of Medical Microbiology, Faculty of Medicine and Faculty of Pharmacy, University of Debrecen, Nagyerdei körút 98, H-4032 Debrecen, Hungary
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University Pharmacy Department of Pharmacy Administration, Faculty of Pharmacy, University of Semmelweis, Hőgyes Endre utca 7-9, H-1092 Budapest, Hungary
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Department of Pharmacology and Pharmacotherapy, University of Debrecen, Nagyerdei körút 98, H-4032 Debrecen, Hungary
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Author to whom correspondence should be addressed.
Pharmaceutics 2019, 11(6), 277; https://doi.org/10.3390/pharmaceutics11060277
Received: 6 May 2019 / Revised: 4 June 2019 / Accepted: 10 June 2019 / Published: 13 June 2019
(This article belongs to the Special Issue 3D Printing of Pharmaceuticals and Drug Delivery Devices)
3D printing is attracting considerable interest for its capacity to produce prototypes and small production runs rapidly. Fused deposit modeling (FDM) was used to produce polyvalent test plates for investigation of the physical, chemical, and in-vitro biological properties of printed materials. The polyvalent test plates (PVTPs) are poly-lactic acid cylinders, 14 mm in diameter and 3 mm in height. The polymer ester backbone was surface modified by a series of ramified and linear oligoamines to increase its hydrophilicity and introduce a positive charge. The chemical modification was verified by FT-IR spectroscopy, showing the introduction of amide and amine functions, and contact angle measurements confirmed increased hydrophilicity. Morphology studies (SEM, optical microscopy) indicated that the modification of PVTP possessed a planar morphology with small pits. Positron annihilation lifetime spectroscopy demonstrated that the polymeric free volume decreased on modification. An MTT-based prolonged cytotoxicity test using Caco-2 cells showed that the PVTPs are non-toxic at the cellular level. The presence of surface oligoamines on the PVTPs reduced biofilm formation by Candida albicans SC5314 significantly. The results demonstrate that 3D printed objects may be modified at their surface by a simple amidation reaction, resulting in a reduced propensity for biofilm colonization and cellular toxicity. View Full-Text
Keywords: fused deposition modeling; polylactic acid; chemical modification; MTT assay; biofilm formation fused deposition modeling; polylactic acid; chemical modification; MTT assay; biofilm formation
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Arany, P.; Róka, E.; Mollet, L.; Coleman, A.W.; Perret, F.; Kim, B.; Kovács, R.; Kazsoki, A.; Zelkó, R.; Gesztelyi, R.; Ujhelyi, Z.; Fehér, P.; Váradi, J.; Fenyvesi, F.; Vecsernyés, M.; Bácskay, I. Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties. Pharmaceutics 2019, 11, 277.

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