Next Article in Journal
Protection Against Rabies Induced by the Non-Replicative Viral Vectors MVA and Ad5 Expressing Rabies Glycoprotein
Next Article in Special Issue
Identification of TRIM21 and TRIM14 as Antiviral Factors Against Langat and Zika Viruses
Previous Article in Journal
Implications of Cognitive Impairment on Antihypertensive Medication Use in HIV
Previous Article in Special Issue
In Vitro System for Studying Ilhéus Virus, a Neglected Arbovirus: Ultrastructural Characterization of Cytopathology, Morphology, and Morphogenesis
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Viruses
Volume 17
Issue 4
10.3390/v17040475
viruses-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Retrospective Study of Arbovirus Circulation in Northeast Brazil in 2019 and 2022: Insights into the Re-Emergence of DENV-3 and the Co-Infection of DENV-1 and CHIKV

by
Sêmilly Suélen da Silva Sousa
1,2,
Ana Cecília Ribeiro Cruz
3,4,
Carine Fortes Aragão
3,
Glennda Juscely Galvão Pereira Cereja
3,
Sandro Patroca da Silva
3,
Raira Maria Morais de Sousa
2,
Murilo Tavares Amorim
3,
Eliana Vieira Pinto da Silva
3,
Bruno Tardelli Diniz Nunes
3 and
Valéria Cristina Soares Pinheiro
1,2,*
1
Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Rede BIONORTE, Universidade Estadual do Maranhão—UEMA, São Luis 65055-310, Brazil
2
Laboratório de Entomologia Médica—LABEM, Universidade Estadual do Maranhão—UEMA, Campus Caxias, Caxias 65604-380, Brazil
3
Instituto Evandro Chagas, Seção de Arbovirologia e Febres Hemorrágicas, Ananindeua 67030-000, Brazil
4
Centro de Ciencias Biológicas e da Saúde, Universidade Estadual do Pará—UEPA, Belém 66087-662, Brazil
*
Author to whom correspondence should be addressed.
Viruses 2025, 17(4), 475; https://doi.org/10.3390/v17040475
Submission received: 20 December 2024 / Revised: 4 March 2025 / Accepted: 18 March 2025 / Published: 26 March 2025
(This article belongs to the Special Issue Advances in Alphavirus and Flavivirus Research, 2nd Edition)
Browse Figures
Versions Notes

Abstract

Arboviruses transmitted by Aedes aegypti cause high number of cases and deaths annually. The aim was to investigate the presence of the presence of Dengue (DENV), Zika (ZIKV) and Chikungunya (CHIKV) viruses in endemic areas of Maranhão, northeastern Brazil. The study was carried out in Caxias, Codó, Peritoró, and São Mateus do Maranhão in 2019 (Caxias) and 2022. The blood samples were subjected to RNA extraction and then tested by RT-qPCR. Cell culture was used to attempt viral isolation and subsequent sequencing. In total, 171 samples were analyzed (32 from 2019, 18.7%) and 72 (42.1%) were found to have arboviruses: 68 (39.7%) from Caxias; 2 (1.1%) from Codó; 1 (0.6%) from Peritoró; and 1 (0.6%) from São Mateus. Overall, 85.3% (n = 58) of the positive samples were infected with DENV-1, 4 (four) (5.9%) with DENV-2 (Caxias), 1 (one) (1.5%) with DENV-3 (Caxias), and in 6 (six) (7.3%) samples CHIKV was detected, with one co-infection of DENV-1 and CHIKV (Caxias). The DENV-1 genotype V and the ECSA genotype of CHIKV were characterized in samples from Caxias. The detection of DENV-1, DENV-2, DENV-3, and more CHIKV in the interior of Maranhão alerts to the importance of virological studies in these areas.
Keywords: arboviruses; coinfection; dengue; chikungunya; molecular surveillance arboviruses; coinfection; dengue; chikungunya; molecular surveillance

Share and Cite

MDPI and ACS Style

Sousa, S.S.d.S.; Cruz, A.C.R.; Aragão, C.F.; Cereja, G.J.G.P.; Silva, S.P.d.; Sousa, R.M.M.d.; Amorim, M.T.; da Silva, E.V.P.; Nunes, B.T.D.; Pinheiro, V.C.S. Retrospective Study of Arbovirus Circulation in Northeast Brazil in 2019 and 2022: Insights into the Re-Emergence of DENV-3 and the Co-Infection of DENV-1 and CHIKV. Viruses 2025, 17, 475. https://doi.org/10.3390/v17040475

AMA Style

Sousa SSdS, Cruz ACR, Aragão CF, Cereja GJGP, Silva SPd, Sousa RMMd, Amorim MT, da Silva EVP, Nunes BTD, Pinheiro VCS. Retrospective Study of Arbovirus Circulation in Northeast Brazil in 2019 and 2022: Insights into the Re-Emergence of DENV-3 and the Co-Infection of DENV-1 and CHIKV. Viruses. 2025; 17(4):475. https://doi.org/10.3390/v17040475

Chicago/Turabian Style

Sousa, Sêmilly Suélen da Silva, Ana Cecília Ribeiro Cruz, Carine Fortes Aragão, Glennda Juscely Galvão Pereira Cereja, Sandro Patroca da Silva, Raira Maria Morais de Sousa, Murilo Tavares Amorim, Eliana Vieira Pinto da Silva, Bruno Tardelli Diniz Nunes, and Valéria Cristina Soares Pinheiro. 2025. "Retrospective Study of Arbovirus Circulation in Northeast Brazil in 2019 and 2022: Insights into the Re-Emergence of DENV-3 and the Co-Infection of DENV-1 and CHIKV" Viruses 17, no. 4: 475. https://doi.org/10.3390/v17040475

APA Style

Sousa, S. S. d. S., Cruz, A. C. R., Aragão, C. F., Cereja, G. J. G. P., Silva, S. P. d., Sousa, R. M. M. d., Amorim, M. T., da Silva, E. V. P., Nunes, B. T. D., & Pinheiro, V. C. S. (2025). Retrospective Study of Arbovirus Circulation in Northeast Brazil in 2019 and 2022: Insights into the Re-Emergence of DENV-3 and the Co-Infection of DENV-1 and CHIKV. Viruses, 17(4), 475. https://doi.org/10.3390/v17040475

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop